Western Blot (WB)
(Western Blot analysis in A549 cell line lysates (35ug/lane) using MBS6009552. This demonstrates that MBS6009552 detected the Actin, alpha1 protein (arrow).)
Rabbit anti-Human Actin, alpha1 Polyclonal Antibody | anti-ACTN1 antibody
Actin, alpha1, CT (ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50
Flow Cytometry: 1:10-50
Immunohistochemistry: 1:10-50
Western Blot (WB)
(Western Blot analysis in A549 cell line lysates (35ug/lane) using MBS6009552. This demonstrates that MBS6009552 detected the Actin, alpha1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in A549 cell line lysates (35ug/lane) using MBS6009552. This demonstrates that MBS6009552 detected the Actin, alpha1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in A549, RD cell line and human placenta lysates (35ug/lane) using MBS6009552. This demonstrates that MBS6009552 detected the Actin, alpha1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in A549, RD cell line and human placenta lysates (35ug/lane) using MBS6009552. This demonstrates that MBS6009552 detected the Actin, alpha1 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS6009552 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6009552 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS6009552 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6009552 for immunohistochemistry.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with A549 cells using MBS6009552 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis with A549 cells using MBS6009552 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 14q24|14q22-q24
Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; extracellular region; dendritic spine; pseudopodium; fascia adherens; Z disc; cytosol; ruffle; cell projection; cytoplasm; plasma membrane; stress fiber; nucleus
Molecular Function: actin filament binding; integrin binding; protein domain specific binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; double-stranded RNA binding; calcium ion binding; vinculin binding
Biological Process: focal adhesion formation; regulation of apoptosis; actin crosslink formation; platelet activation; actin filament bundle formation; extracellular matrix organization and biogenesis; platelet degranulation; negative regulation of cell motility; blood coagulation
Disease: Bleeding Disorder, Platelet-type, 15
Research Articles on ACTN1
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Product Notes
The ACTN1 actn1 (Catalog #AAA6009552) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Actin, alpha1, CT (ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Actin, alpha1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunofluorescence: 1:10-50 Flow Cytometry: 1:10-50 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the ACTN1 actn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, alpha1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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