Rabbit anti-Human, mouse PACSIN3 Polyclonal Antibody | anti-PACSIN3 antibody

PACSIN3 Antibody (N-term)

Cat. Num. AAA9208842

• Gene Names: PACSIN3; SDPIII
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PACSIN3; Polyclonal Antibody; PACSIN3 Antibody (N-term); Protein kinase C and casein kinase substrate in neurons protein 3; SH3 domain-containing protein 6511; anti-PACSIN3 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PACSIN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PACSIN3.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 424

• Applicable Applications for anti-PACSIN3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-PACSIN3 Pab is used in Western blot to detect PACSIN3 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(PACSIN3 Antibody (M1) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PACSIN3 antibody detected the PACSIN3 protein (arrow).)

Western Blot (WB)

(Western blot analysis of PACSIN3 (arrow) using rabbit polyclonal PACSIN3 Antibody (M1). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PACSIN3 gene.)

Related Product Information for anti-PACSIN3 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-PACSIN3 antibody

Signal Transduction

References

Howard, L., et al., J. Biol. Chem. 274(44):31693-31699 (1999). Modregger, J., et al., J. Cell. Sci. 113 Pt 24, 4511-4521 (2000). Sumoy, L., et al., Gene 262 (1-2), 199-205 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 296923797
• NCBI GeneID: 29763
• NCBI Accession #: NP_001171903.1; NP_001171904.1; NP_057307.2
• NCBI GenBank Nucleotide #: NM_001184974.1
• UniProt Accession #: Q9UKS6; Q9H331; Q9NWV9; A6NH84
• Molecular Weight: 48487
• NCBI Official Full Name: protein kinase C and casein kinase substrate in neurons protein 3
• NCBI Official Synonym Full Names: protein kinase C and casein kinase substrate in neurons 3
• NCBI Official Symbol: PACSIN3
• NCBI Official Synonym Symbols: SDPIII
• NCBI Protein Information: protein kinase C and casein kinase substrate in neurons protein 3
• UniProt Protein Name: Protein kinase C and casein kinase substrate in neurons protein 3
• Protein Family: Protein kinase C and casein kinase II substrate protein
• UniProt Gene Name: PACSIN3
• UniProt Entry Name: PACN3_HUMAN

NCBI Description

This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

PACSIN3: May play a role in vesicle formation and transport. Belongs to the PACSIN family.

Protein type: Adaptor/scaffold; Vesicle

Chromosomal Location of Human Ortholog: 11p12-p11.12

Cellular Component: nucleoplasm; membrane; cytoplasm; plasma membrane; endosome

Molecular Function: protein binding; cytoskeletal protein binding; phospholipid binding; calcium channel inhibitor activity; lipid binding

Biological Process: negative regulation of calcium ion transport; negative regulation of endocytosis; regulation of endocytosis; cytoskeleton organization and biogenesis; endocytosis; positive regulation of membrane protein ectodomain proteolysis