Chicken Lysozyme (LZM) ELISA Kit | LZM elisa kit
Chicken Lysozyme (LZM) Mini Samples ELISA Kit
Gene Names
LYZ; LYZC
Reactivity
Chicken
Synonyms
Lysozyme (LZM); Chicken Lysozyme (LZM) Mini Samples ELISA Kit; LYZ; Renal Amyloidosis; N-Acetylmuramide Glycanhydrolase; Muramidase; 1; 4-beta-N-acetylmuramidase C; LZM elisa kit
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of lysozyme. No significant cross-reactivity or interference between lysozyme and analogues was observed.
Sequence Length
147
Samples
Gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.625-40ng/mL
Sensitivity
<0.293ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level lysozyme were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level lysozyme were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LZM elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of lysozyme in gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to lysozyme. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to lysozyme. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain lysozyme, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of lysozyme in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to lysozyme. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to lysozyme. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain lysozyme, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of lysozyme in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for LZM elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,239 Da
NCBI Official Full Name
lysozyme C
NCBI Official Synonym Full Names
lysozyme (renal amyloidosis)
NCBI Official Symbol
LYZ
NCBI Official Synonym Symbols
LYZC
NCBI Protein Information
lysozyme C
UniProt Protein Name
Lysozyme C
UniProt Gene Name
LYZ
Uniprot Description
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.
