Rat GATA4 (GATA Binding Protein 4) CLIA Kit | GATA4 clia kit

Rat GATA4 (GATA Binding Protein 4) CLIA Kit

Cat. Num. AAA2531282

• Gene Names: GATA4; TOF; ASD2; VSD1; TACHD
• Reactivity: Rat
• Synonyms: GATA4 (GATA Binding Protein 4); Rat GATA4 (GATA Binding Protein 4) CLIA Kit; GATA4 clia kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant GATA4. No significant cross-reactivity or interference between GATA4 and analogues was observed.
• Sequence Length: 442

• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GATA4 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of GATA4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to GATA4. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GATA4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GATA4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of GATA4. You can calculate the concentration of GATA4 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 219518812
• NCBI GeneID: 2626
• Molecular Weight: 44,665 Da
• NCBI Official Full Name: GATA binding protein 4
• NCBI Official Synonym Full Names: GATA binding protein 4
• NCBI Official Symbol: GATA4
• NCBI Official Synonym Symbols: TOF; ASD2; VSD1; TACHD
• NCBI Protein Information: transcription factor GATA-4
• UniProt Protein Name: Transcription factor GATA-4
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA4
• UniProt Entry Name: GATA4_HUMAN

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

GATA4: a conserved and ubiquitous member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. May regulate a set of cardiac-specific genes and play a crucial role in cardiogenesis. Defects are a cause of atrial septal defect 2 (ASD2). ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or noncardiac abnormalities.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 8p23.1-p22

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; transcription activator binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; protein kinase binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cardiac muscle hypertrophy; negative regulation of autophagy; embryonic heart tube anterior/posterior pattern formation; positive regulation of transcription, DNA-dependent; Sertoli cell differentiation; Wnt receptor signaling pathway through beta-catenin; response to vitamin A; BMP signaling pathway; cell-cell signaling; regulation of transcription, DNA-dependent; embryonic digestive tract morphogenesis; embryonic foregut morphogenesis; anatomical structure formation; heart looping; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; cell fate commitment; response to retinoic acid; in utero embryonic development; male gonad development; gastrulation with mouth forming second; positive regulation of angiogenesis; response to mechanical stimulus; response to estrogen stimulus; endoderm formation; positive regulation of cardioblast differentiation; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; blood coagulation; cell development; endoderm development

Disease: Ventricular Septal Defect 1; Atrial Septal Defect 2; Tetralogy Of Fallot; Atrioventricular Septal Defect 4; Testicular Anomalies With Or Without Congenital Heart Disease