Rabbit anti-Human, Mouse ACSS1 Polyclonal Antibody | anti-ACSS1 antibody

ACSS1 Polyclonal Antibody

Cat. Num. AAA3002833

• Gene Names: ACSS1; ACAS2L; ACECS1; AceCS2L
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ACSS1; Polyclonal Antibody; ACSS1 Polyclonal Antibody; ACAS2L; KIAA1846; Acetyl-coenzyme A synthetase 2-like; mitochondrial; Acetate--CoA ligase 2; Acetyl-CoA synthetase 2; AceCS2; Acyl-CoA synthetase short-chain family member 1; anti-ACSS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: ACSS1 Polyclonal Antibody detects endogenous levels of ACSS1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-ACSS1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; IHC: 1/100-1/300; ELISA: 1/40,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human ACSS1. AA range: 611-660
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of various cells using ACSS1 Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of HT29 cells using ACSS1 Polyclonal Antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ACSS1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Related Product Information for anti-ACSS1 antibody

Background: acyl-CoA synthetase short-chain family member 1(ACSS1) Homo sapiens. This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

NCBI and Uniprot Product Information

• NCBI GI #: 358248205
• NCBI GeneID: 84532
• NCBI Accession #: NP_001239604.1
• NCBI GenBank Nucleotide #: NM_001252675.1
• UniProt Accession #: Q9NUB1; Q5TF42; Q8IV99; Q8N234; Q96JI1; Q96JX6; Q9NU28; B3KXL2; B4DJZ3; D3DW48; F5H6F4; F8W7Y1
• Molecular Weight: 74,857 Da
• NCBI Official Full Name: acetyl-coenzyme A synthetase 2-like, mitochondrial isoform 2
• NCBI Official Synonym Full Names: acyl-CoA synthetase short-chain family member 1
• NCBI Official Symbol: ACSS1
• NCBI Official Synonym Symbols: ACAS2L; ACECS1; AceCS2L
• NCBI Protein Information: acetyl-coenzyme A synthetase 2-like, mitochondrial; acetate--CoA ligase 2
• UniProt Protein Name: Acetyl-coenzyme A synthetase 2-like, mitochondrial
• Protein Family: Acetyl-coenzyme A synthetase
• UniProt Gene Name: ACSS1
• UniProt Synonym Gene Names: ACAS2L; KIAA1846; AceCS2
• UniProt Entry Name: ACS2L_HUMAN

NCBI Description

This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

ACSS1: Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions. Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2). Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.2.1.1; Carbohydrate Metabolism - pyruvate; Ligase; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: mitochondrial matrix

Molecular Function: protein binding; acetate-CoA ligase activity; ATP binding; AMP binding

Biological Process: acetyl-CoA biosynthetic process from acetate; xenobiotic metabolic process; acetyl-CoA biosynthetic process; propionate biosynthetic process; ethanol oxidation; acetate biosynthetic process