Western Blot (WB)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse ACP3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Acid Phosphatase 3, Prostatic (ACP3) Polyclonal Antibody | anti-ACP3 antibody
Polyclonal Antibody to Acid Phosphatase 3, Prostatic (ACP3)
MGHHHHHHSGS-PITES SWPQGFGQLT QWGMEQHYEL GSYIRKRYGR FLNDTYKHDQ IYIRSTDVDR TLMSAMTNLA ALFPPEGISI WNPRLLWQPI PVHTVSLSED RLLYLPFRDC PRFEELKSET LESEEFLKRL HPYKSFLDTL SSLSGFDDQD LFGIWSKVYD PLFCE
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse ACP3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human MCF7 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse ACP3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
Uniprot Description
ACPP: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Belongs to the histidine acid phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.2; EC 3.1.3.5; Motility/polarity/chemotaxis; Phosphatase
Chromosomal Location of Human Ortholog: 9|9 F1
Cellular Component: apical part of cell; extracellular region; extracellular space; filopodium; Golgi cisterna; integral component of membrane; intracellular; lysosomal membrane; lysosome; membrane; multivesicular body; nucleus; plasma membrane; secretory granule; vesicle membrane
Molecular Function: 5'-nucleotidase activity; acid phosphatase activity; choline binding; hydrolase activity; identical protein binding; phosphoric monoester hydrolase activity; protein homodimerization activity; thiamine phosphate phosphatase activity
Biological Process: adenosine metabolic process; dephosphorylation; nucleotide metabolic process; positive regulation of adenosine receptor signaling pathway; protein homotetramerization; purine nucleobase metabolic process; regulation of sensory perception of pain; thiamin metabolic process
Research Articles on ACP3
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Product Notes
The ACP3 acpp (Catalog #AAA2005636) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Acid Phosphatase 3, Prostatic (ACP3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Acid Phosphatase 3, Prostatic (ACP3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ACP3 acpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S-PITES SWPQGFGQLT QWGMEQHYEL GSYIRKRYGR FLNDTYKHDQ IYIRSTDVDR TLMSAMTNLA ALFPPEGISI WNPRLLWQPI PVHTVSLSED RLLYLPFRDC PRFEELKSET LESEEFLKRL HPYKSFLDTL SSLSGFDDQD LFGIWSKVYD PLFCE. It is sometimes possible for the material contained within the vial of "Acid Phosphatase 3, Prostatic (ACP3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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