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SDS-PAGE

Prostatic Acid Phosphatase / ACPP Recombinant Protein | ACPP recombinant protein

Recombinant Mouse Prostatic Acid Phosphatase / ACPP Protein (His tag)

Gene Names
Acpp; Lap; PAP; FRAP; Ppal; 5'-NT; A030005E02Rik
Purity
> 98 % as determined by SDS-PAGE
Synonyms
Prostatic Acid Phosphatase / ACPP; Recombinant Mouse Prostatic Acid Phosphatase / ACPP Protein (His tag); 9.104100899-104272570.1; 5'-NT; A030005E02Rik; FRAP; Lap; PAP; Ppal; ACPP recombinant protein
Ordering
For Research Use Only!
Host
Human Cells
Purity/Purification
> 98 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
381
Application Notes
The recombinant mouse ACPP consists of 361 amino acids and predicts a molecular mass of 42 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACPP is approximately 47 KDa as a result of glycosylation.
Predicted N Terminal
Lys 32
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for ACPP recombinant protein
Background: Prostatic acid phosphatase (PAP, or ACPP), also known as prostatic specific acid phosphatase (PSAP), is an enzyme produced by the prostate. As a non-specific phosphomonoesterase, Prostatic acid phosphatase synthetized and secreted into seminal plasma under androgenic control. The enzyme is a dimer of molecular weight around 100 kDa. Prostatic acid phosphatase is a clinically important protein for its relevance as a biomarker of prostate carcinoma. Furthermore, it has a potential role in fertilization. The major action of PAP is to dephosphorylate macromolecules with the help of catalytic residues (His (12) and Asp (258)) that are located in the cleft between two domains. Cellular prostatic acid phosphatase (cPAcP), an authentic tyrosine phosphatase, is proposed to function as a negative growth regulator of prostate cancer (PCa) cells in part through its dephosphorylation of ErbB-2. cPAcP functions as a neutral protein tyrosine phosphatase (PTP) in prostate cancer cells and dephosphorylates HER-2/ErbB-2/Neu (HER-2: human epidermal growth factor receptor-2) at the phosphotyrosine (p-Tyr) residues. Injection of the secretory isoform of PAP has potent antinociceptive effects in mouse models of chronic pain. This enzyme exhibits ecto-5'-nucleotidase activity, is widely distributed, and implicated in the formation of chronic pain. Additionally, PAP could be a target molecule in specific immunotherapy for patients with nonprostate adenocarcinomas including colon and gastric cancers.

Description: A DNA sequence encoding the mouse ACPP isoform 1 (Q8CE08-1) (Met1-Arg 381) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,863 Da
NCBI Official Full Name
prostatic acid phosphatase isoform 2
NCBI Official Synonym Full Names
acid phosphatase, prostate
NCBI Official Symbol
Acpp
NCBI Official Synonym Symbols
Lap; PAP; FRAP; Ppal; 5'-NT; A030005E02Rik
NCBI Protein Information
prostatic acid phosphatase
UniProt Protein Name
Prostatic acid phosphatase
UniProt Gene Name
Acpp
UniProt Synonym Gene Names
5'-NT; FRAP; TMPase

Uniprot Description

ACPP: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Belongs to the histidine acid phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.2; EC 3.1.3.5; Motility/polarity/chemotaxis; Phosphatase

Chromosomal Location of Human Ortholog: 9|9 F1

Cellular Component: apical part of cell; extracellular space; filopodium; Golgi cisterna; integral to membrane; intracellular; lysosomal membrane; multivesicular body; nucleus; plasma membrane; secretory granule; vesicle membrane

Molecular Function: 5'-nucleotidase activity; acid phosphatase activity; choline binding; identical protein binding; phosphoric monoester hydrolase activity; protein homodimerization activity; thiamin phosphate phosphatase activity

Biological Process: adenosine metabolic process; dephosphorylation; nucleotide metabolic process; positive regulation of adenosine receptor signaling pathway; purine base metabolic process; regulation of sensory perception of pain; thiamin metabolic process

Research Articles on ACPP

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Product Notes

The ACPP acpp (Catalog #AAA2546690) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse ACPP consists of 361 amino acids and predicts a molecular mass of 42 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACPP is approximately 47 KDa as a result of glycosylation. Researchers should empirically determine the suitability of the ACPP acpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prostatic Acid Phosphatase / ACPP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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