Western Blot (WB)
(Western blot analysis of Cytochrome P450 1A1/2 expression in MCF7 (A), HeLa (B) whole cell lysates.)
Rabbit Cytochrome P450 1A1/2 Polyclonal Antibody | anti-CYP1A1 antibody
Anti-Cytochrome P450 1A1/2 Antibody
Western Blot (WB)
(Western blot analysis of Cytochrome P450 1A1/2 expression in MCF7 (A), HeLa (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Cytochrome P450 1A1/2 expression in MCF7 (A), HeLa (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Cytochrome P450 1A1/2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Cytochrome P450 1A1/2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Cytochrome P450 1A1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Cytochrome P450 1A1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq, Jul 2008]
Uniprot Description
CYP1A1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.
Protein type: EC 1.14.14.1; Oxidoreductase; Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol
Chromosomal Location of Human Ortholog: 15q24.1
Cellular Component: endoplasmic reticulum membrane; mitochondrion
Molecular Function: flavonoid 3'-monooxygenase activity; oxidoreductase activity, acting on diphenols and related substances as donors; enzyme binding; iron ion binding; heme binding; steroid hydroxylase activity; oxidoreductase activity; oxygen binding; demethylase activity
Biological Process: coumarin metabolic process; response to nematode; response to arsenic; response to lipopolysaccharide; cellular lipid metabolic process; response to vitamin A; response to antibiotic; embryonic development ending in birth or egg hatching; flavonoid metabolic process; porphyrin metabolic process; response to wounding; arachidonic acid metabolic process; drug metabolic process; aging; response to drug; response to food; insecticide metabolic process; camera-type eye development; response to iron(III) ion; response to virus; epoxygenase P450 pathway; response to herbicide; vitamin D metabolic process; dibenzo-p-dioxin catabolic process; cell proliferation; response to hyperoxia; gut development; xenobiotic metabolic process; maternal process involved in parturition; 9-cis-retinoic acid biosynthetic process; response to hypoxia; amine metabolic process; hydrogen peroxide biosynthetic process
Research Articles on CYP1A1
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Product Notes
The CYP1A1 cyp1a1 (Catalog #AAA821638) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytochrome P450 1A1/2 Antibody reacts with Human, Mouse, Rat, Dog, Monkey, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 1A1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence/Immunocytochemistry (IF/ICC), Immunoprecipitation (IP). WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the CYP1A1 cyp1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 1A1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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