Western Blot (WB)
(Western blot analysis of extracts from rat heart, using ACADS antibody. Lane 1 was treated with the blocking peptide.)
Rabbit ACADS Polyclonal Antibody | anti-ACADS antibody
ACADS Antibody
Gene Names
ACADS; SCAD; ACAD3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ACADS; Polyclonal Antibody; ACADS Antibody; ACAD3; ACADS_HUMAN; Acyl Coenzyme A dehydrogenase; C2 to C3 short chain; Acyl-CoA dehydrogenase; short chain; Acyl-Coenzyme A dehydrogenase; AI196007; Bcd-1; Bcd1; Butyryl CoA dehydrogenase; Butyryl-CoA dehydrogenase; EC 1.3.99.2; mitochondrial; SCAD; Short chain acyl CoA dehydrogenase; Short-chain specific acyl-CoA dehydrogenase; Unsaturated acyl CoA reductase; anti-ACADS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
ACADS antibody detects endogenous levels of ACADS.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
412
Applicable Applications for anti-ACADS antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human ACADS.
Subcellular Location
Mitochondrion Matrix.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from rat heart, using ACADS antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from rat heart, using ACADS antibody. Lane 1 was treated with the blocking peptide.)
Immunohistochemistry (IHC)
(MBS9603413 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603413 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of COS-7 cells, using ACADS antibody.)
Western Blot (WB)
(Western blot analysis of extracts of COS-7 cells, using ACADS antibody.)
Related Product Information for anti-ACADS antibody
Function: Introduces a double bond at position 2 in saturated acyl-CoA's of short chain length, i.e. less than 6 carbon atoms.
Subunit Structure: Homotetramer.
Similarity: Belongs to the acyl-CoA dehydrogenase family.
Subunit Structure: Homotetramer.
Similarity: Belongs to the acyl-CoA dehydrogenase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 42 kDa
Predicted: 45 kDa
Predicted: 45 kDa
NCBI Official Full Name
short-chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1
NCBI Official Synonym Full Names
acyl-CoA dehydrogenase short chain
NCBI Official Symbol
ACADS
NCBI Official Synonym Symbols
SCAD; ACAD3
NCBI Protein Information
short-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Protein Name
Short-chain specific acyl-CoA dehydrogenase, mitochondrial
Protein Family
UniProt Gene Name
ACADS
UniProt Synonym Gene Names
SCAD
NCBI Description
This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014]
Uniprot Description
Introduces a double bond at position 2 in saturated acyl-CoA's of short chain length, i.e. less than 6 carbon atoms.
Research Articles on ACADS
Similar Products
Product Notes
The ACADS acads (Catalog #AAA9603413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACADS Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ACADS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ACADS acads for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACADS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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