Rabbit anti-Human SCAD / ACADS Polyclonal Antibody | anti-SCAD antibody

Rabbit Polyclonal to Human SCAD / ACADS

Cat. Num. AAA247943

• Gene Names: ACADS; SCAD; ACAD3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: SCAD / ACADS; Polyclonal Antibody; Rabbit Polyclonal to Human SCAD / ACADS; Anti-SCAD / ACADS Antibody IHC-plus; ACADS; ACAD3; Butyryl-CoA dehydrogenase; SCAD; Unsaturated acyl-CoA reductase; Human SCAD; anti-SCAD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human ACADS. Predicted cross-reactivity based on amino acid sequence homology: mouse (84%), rat (84%), bovine (84%), pig (83%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
• Sequence Length: 412

• Applicable Applications for anti-SCAD antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:500 - 1:3000)
• Target Species: Human
• Immunogen Description: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 104 of Human SCAD.
• Immunogen Type: Recombinant protein
• Immunogen: SCAD / ACADS antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 104 of Human SCAD.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ACADS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Sample(30 g of whole cell lysate). A: MOLT4. 12% SDS PAGE. ACADS antibody diluted at 1:500.)

Related Product Information for anti-SCAD antibody

The nuclear ACADS gene encodes a mitochondrially-targeted protein product. Acyl-Coenzyme A dehydrogenase, short-chain specific, catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Clinical phenotypes, described elsewhere, are associated with ACADS hereditary deficiency.

NCBI and Uniprot Product Information

• NCBI GI #: 4557233
• NCBI GeneID: 35
• NCBI Accession #: NP_000008.1
• NCBI GenBank Nucleotide #: NM_000017.3
• UniProt Accession #: P16219; P78331
• Molecular Weight: 44,297 Da
• NCBI Official Full Name: short-chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase, C-2 to C-3 short chain
• NCBI Official Symbol: ACADS
• NCBI Official Synonym Symbols: SCAD; ACAD3
• NCBI Protein Information: short-chain specific acyl-CoA dehydrogenase, mitochondrial; butyryl-CoA dehydrogenase; unsaturated acyl-CoA reductase; acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain; mitochondrial short-chain specific acyl-CoA dehydrogenase
• UniProt Protein Name: Short-chain specific acyl-CoA dehydrogenase, mitochondrial
• UniProt Gene Name: ACADS
• UniProt Synonym Gene Names: SCAD
• UniProt Entry Name: ACADS_HUMAN

NCBI Description

This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

ACADS: Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD). It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults. Belongs to the acyl-CoA dehydrogenase family.

Protein type: EC 1.3.8.1; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial membrane; nucleus

Molecular Function: acyl-CoA dehydrogenase activity; butyryl-CoA dehydrogenase activity; FAD binding; acyl-CoA binding

Biological Process: response to starvation; fatty acid beta-oxidation; response to glucocorticoid stimulus; cellular lipid metabolic process; fatty acid beta-oxidation using acyl-CoA dehydrogenase; protein homotetramerization; butyrate catabolic process

Disease: Acyl-coa Dehydrogenase, Short-chain, Deficiency Of