Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACADS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : LnCap cell lysate)
Mouse anti-Human ACADS Monoclonal Antibody | anti-ACADS antibody
ACADS antibody
Gene Names
ACADS; SCAD; ACAD3
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
ACADS; Monoclonal Antibody; ACADS antibody; Acyl-Coenzyme A dehydrogenase; ACAD3; SCAD; Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase; C2 to C3 short chain; Acyl-CoA dehydrogenase; short chain; AI196007; Bcd-1; Bcd1; Butyryl CoA dehydrogenase; EC 1.3.99.2; Short chain acyl CoA dehydrogenase; unsaturated acyl CoA reductase; Short-chain specific acyl-CoA dehydrogenase; mitochondrial.; anti-ACADS antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT8B10
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
412
Applicable Applications for anti-ACADS antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human ACADS (25-412aa) purified from E. coli
Clone Note
Anti-human ACADS mAb, clone AT8B10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACADS protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACADS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : LnCap cell lysate)
Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACADS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : LnCap cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of ACADS in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ACADS in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of ACADS in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ACADS in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-ACADS antibody
ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency).
Product Categories/Family for anti-ACADS antibody
References
McAndrew RP., et al. (2008) J Biol Chem. 283 (14): 9435-43. ; Tafti M., et al. (2003) Nat Genet. 34 (3): 320-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,297 Da
NCBI Official Full Name
Acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
NCBI Official Synonym Full Names
acyl-CoA dehydrogenase short chain
NCBI Official Symbol
ACADS
NCBI Official Synonym Symbols
SCAD; ACAD3
NCBI Protein Information
short-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Protein Name
Short-chain specific acyl-CoA dehydrogenase, mitochondrial
Protein Family
UniProt Gene Name
ACADS
UniProt Synonym Gene Names
SCAD
NCBI Description
This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014]
Uniprot Description
Introduces a double bond at position 2 in saturated acyl-CoA's of short chain length, i.e. less than 6 carbon atoms.
Research Articles on ACADS
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Product Notes
The ACADS acads (Catalog #AAA200465) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACADS antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACADS can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the ACADS acads for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACADS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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