ACADS recombinant protein

ACADS, 25-412aa, Human, His tag, E Coli

Cat. Num. AAA203517

• Gene Names: ACADS; SCAD; ACAD3
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ACADS; 25-412aa; Human; His tag; E Coli; Acyl-Coenzyme A dehydrogenase; ACAD3; SCAD; Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase; C2 to C3 short chain; Acyl-CoA dehydrogenase; short chain; AI196007; Bcd-1; Bcd1; Butyryl CoA dehydrogenase; EC 1.3.99.2; Short chain acyl CoA dehydrogenase; unsaturated acyl CoA reductase; Short-chain specific acyl-CoA dehydrogenase; mitochondrial.; ACADS recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT 0.1 M NaCl, and 20% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MLHTIYQSVE LPETHQMLLQ TCRDFAEKEL FPIAAQVDKE HLFPAAQVKK MGGLGLLAMD VPEELGGAGL DYLAYAIAME EISRGCASTG VIMSVNNSLY LGPILKFGSK EQKQAWVTPF TSGDKIGCFA LSEPGNGSDA GAASTTARAE GDSWVLNGTK AWITNAWEAS AAVVFASTDR ALQNKSISAF LVPMPTPGLT LGKKEDKLGI RGSSTANLIF EDCRIPKDSI LGEPGMGFKI AMQTLDMGRI GIASQALGIA QTALDCAVNY AENRMAFGAP LTKLQVIQFK LADMALALES ARLLTWRAAM LKDNKKPFIK EAAMAKLAAS EAATAISHQA IQILGGMGYV TEMPAERHYR DARITEIYEG TSEIQRLVIA GHLLRSYRS
• Sequence Length: 412

• Applicable Applications for ACADS recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ACADS recombinant protein

ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency). Recombinant human ACADS protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ACADS recombinant protein

Redox Proteins

References

McAndrew RP., et al. (2008) J Biol Chem. 283 (14):9435-43. ; Tafti M., et al. (2003) Nat Genet. 34 (3):320-5.;

NCBI and Uniprot Product Information

• NCBI GI #: 19684166
• NCBI GeneID: 35
• Molecular Weight: 44.0 kDa (409aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: Acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase short chain
• NCBI Official Symbol: ACADS
• NCBI Official Synonym Symbols: SCAD; ACAD3
• NCBI Protein Information: short-chain specific acyl-CoA dehydrogenase, mitochondrial
• UniProt Protein Name: Short-chain specific acyl-CoA dehydrogenase, mitochondrial
• Protein Family: Short-chain specific acyl-CoA dehydrogenase
• UniProt Gene Name: ACADS
• UniProt Synonym Gene Names: SCAD

NCBI Description

This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

Introduces a double bond at position 2 in saturated acyl-CoA's of short chain length, i.e. less than 6 carbon atoms.