Immunohistochemistry (IHC)
(MBS9607574 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit ABCC4 Polyclonal Antibody | anti-ABCC4 antibody
ABCC4 Antibody
Gene Names
ABCC4; MRP4; MOATB; MOAT-B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ABCC4; Polyclonal Antibody; ABCC4 Antibody; ABCC 4; ATP binding cassette sub family C (CFTR/MRP) member 4; ATP binding cassette sub family C member 4; ATP-binding cassette sub-family C member 4; bA464I2.1 (ATP binding cassette; sub-family C (CFTR/MRP) member 4); bA464I2.1; Canalicular multispecific organic anion transporter; Canalicular multispecific organic anion transporter ABC superfamily; EST170205; MOAT B; MOAT-B; MOATB; MRP 4; MRP/cMOAT related ABC transporter; MRP/cMOAT-related ABC transporter; MRP4_HUMAN; Multi specific organic anion transporter B; Multi-specific organic anion transporter B; Multidrug resistance associated protein 4; Multidrug resistance-associated protein 4; OTTHUMP00000018560; anti-ABCC4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ABCC4 antibody detects endogenous levels of total ABCC4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
859
Applicable Applications for anti-ABCC4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human ABCC4
Subcellular Location
Membrane.
Tissue Specificity
Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
Predicted Cross Reactivity
Rabbit
Similarity
Rabbit (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9607574 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9607574 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of kindney, using ABCC4 antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts of kindney, using ABCC4 antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-ABCC4 antibody
Description: ABCC4 is a member of the ATP-binding Cassette (ABC) transporter family. ABC proteins transport various molecules across cellular membranes by utilizing the energy generated from ATP hydrolysis. There are seven subfamilies of ABC proteins: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White (1). ABCC4 belongs to the MRP subfamily, which is involved in multi-drug resistance, hence it is also named MRP4. ABCC4 is widely expressed in cells and tissues including prostate, kidney proximal tubules, astrocytes and capillary endothelial cells of the brain, platelets, and many cancer cell lines (2-4). ABCC4 mediates efflux transport of a wide variety of endogenous and xenobiotic organic anionic compounds (5). The diversity of substrates determines the biological functions of ABCC4. It regulates cAMP levels in human leukemia cells, thereby controlling the proliferation and differentiation of leukemia cells (6). ABCC4 also enables COX deficient pancreatic cancer cells to obtain exogenous prostagladins (7). Researchers have shown that ABCC4 expression is elevated in drug resistant cancer cells, which makes it a potential target for cancer therapy (8, 9). ABCC4 localizes to both plasma membrane and intracellular membranous structures (10). Investigators have also implicated ABCC4 in the pathogenesis of Kawasaki desease, a genetic childhood disease characterized by vasculitis (11).
Function: May be an organic anion pump relevant to cellular detoxification.
Subunit Structure: Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain).
Similarity: Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Function: May be an organic anion pump relevant to cellular detoxification.
Subunit Structure: Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain).
Similarity: Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 97 kDa
Predicted: 150 kDa
Predicted: 150 kDa
NCBI Official Full Name
multidrug resistance-associated protein 4 isoform 2
NCBI Official Synonym Full Names
ATP binding cassette subfamily C member 4
NCBI Official Symbol
ABCC4
NCBI Official Synonym Symbols
MRP4; MOATB; MOAT-B
NCBI Protein Information
multidrug resistance-associated protein 4
UniProt Protein Name
Multidrug resistance-associated protein 4
Protein Family
UniProt Gene Name
ABCC4
UniProt Synonym Gene Names
MRP4; MOAT-B
NCBI Description
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Uniprot Description
May be an organic anion pump relevant to cellular detoxification.
Research Articles on ABCC4
Similar Products
Product Notes
The ABCC4 abcc4 (Catalog #AAA9607574) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ABCC4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ABCC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ABCC4 abcc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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