Rabbit anti-Human, Mouse A2ML1 Polyclonal Antibody | anti-A2ML1 antibody

A2ML1, NT (A2ML1, CPAMD9, Alpha-2-macroglobulin-like protein 1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9)

Cat. Num. AAA648921

• Gene Names: A2ML1; CPAMD9
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: A2ML1; Polyclonal Antibody; NT (A2ML1; CPAMD9; Alpha-2-macroglobulin-like protein 1; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9); Anti -A2ML1; anti-A2ML1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-A2ML1 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50
• Immunogen: A2ML1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352~381 amino acids from the N-terminal region of human A2ML1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of A2ML1 Antibody (N-term) in mouse lung tissue lysates (35ug/lane). A2ML1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(A2ML1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-A2ML1 antibody

A2ML1 is a new member of the alpha 2-macroglobulin protease inhibitor family, and shares most the characteristics of this family. It is the first family member detected in the epidermis and may play an important role during desquamation by inhibiting extracellular proteases.

Product Categories/Family for anti-A2ML1 antibody

Antibodies; Abs to Protease Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 539846446
• NCBI GeneID: 144568
• NCBI Accession #: NP_001269353.1
• NCBI GenBank Nucleotide #: NM_001282424.1
• UniProt Accession #: A8K2U0; Q2M224; Q6ZW52; Q6ZW53; Q8N1M4; Q96LQ8; B5MDD1
• Molecular Weight: 161,107 Da
• NCBI Official Full Name: alpha-2-macroglobulin-like protein 1 isoform 2
• NCBI Official Synonym Full Names: alpha-2-macroglobulin-like 1
• NCBI Official Symbol: A2ML1
• NCBI Official Synonym Symbols: CPAMD9
• NCBI Protein Information: alpha-2-macroglobulin-like protein 1; C3 and PZP-like, alpha-2-macroglobulin domain containing 9
• UniProt Protein Name: Alpha-2-macroglobulin-like protein 1
• Protein Family: Alpha-2-macroglobulin-like protein
• UniProt Gene Name: A2ML1
• UniProt Synonym Gene Names: CPAMD9
• UniProt Entry Name: A2ML1_HUMAN

NCBI Description

This gene encodes a member of the alpha-macroglobulin superfamily. The encoded protein acts as an inhibitor for several proteases, and has been reported as the p170 antigen recognized by autoantibodies in the autoimmune disease paraneoplastic pemphigus (PNP; PMID: 20805888). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase

By similarity. Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases. Ref.5

Subunit structure: Monomer. Ref.5

Subcellular location: Secreted Ref.5.

Tissue specificity: In the epidermis, expressed predominantly in the granular layer at the apical edge of keratinocytes (at protein level). Also detected in placenta, testis and thymus but not in epithelia of kidney, lung, small intestine or colon. Ref.5

Developmental stage: Up-regulated during keratinocyte differentiation. Ref.5

Sequence similarities: Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Sequence caution: The sequence BAB71612.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC04793.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC85653.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC85654.1 differs from that shown. Reason: Erroneous initiation.