Rabbit anti-Human, Mouse GGA1 Polyclonal Antibody | anti-GGA1 antibody

GGA1, NT (GGA1, ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, Golgi-localized, gamma ear-containing, ARF-binding protein 1) (AP)

Cat. Num. AAA6302050

• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: GGA1; Polyclonal Antibody; NT (GGA1; ADP-ribosylation factor-binding protein GGA1; Gamma-adaptin-related protein 1; Golgi-localized; gamma ear-containing; ARF-binding protein 1) (AP); ARF-binding protein 1; anti-GGA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-GGA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human GGA1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-GGA1 Pab is used in Western blot to detect GGA1 in mouse kidney tissue lysate (lane 1) and Jurkat cell lysate (lane 2).)

Immunostaining (IS)

(A, N2a cells cotransfected with APP770-V5 and GGA1-myc or empty vector were immunostained for APP (Alexa488; green), GGA1 (Cy5; blue), and the Golgi marker GM130 (Cy3; red). B, The same transfection was stained for the endosomal marker EEA1 (Cy3). C, To assess cell-surface localization, APP695-GFP and GGA1-myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5). (J. Neurosci. 2006 Sep 27;26(39):9913-9922))

Related Product Information for anti-GGA1 antibody

GGA1 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.

Product Categories/Family for anti-GGA1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Zhai, P., et al., Biochemistry 42(47):13901-13908 (2003). Zhu, G., et al., Biochemistry 42(21):6392-6399 (2003). Shiba, T., et al., Nat. Struct. Biol. 10(5):386-393 (2003). Suer, S., et al., Proc. Natl. Acad. Sci. U.S.A. 100(8):4451-4456 (2003). Shiba, T., et al., Nature 415(6874):937-941 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 14548066
• NCBI GeneID: 26088
• UniProt Accession #: Q9UJY5; Q5TG07; Q86YA9; Q8NCS6; Q9BW94; Q9UG00; Q9UGW0; Q9UGW1; A8K3D3; B0QYR7
• Molecular Weight: 9,969 Da
• NCBI Official Full Name: ADP-ribosylation factor-binding protein GGA1
• NCBI Official Synonym Full Names: golgi-associated, gamma adaptin ear containing, ARF binding protein 1
• NCBI Official Symbol: GGA1
• NCBI Protein Information: ADP-ribosylation factor-binding protein GGA1; gamma-adaptin related protein 1; gamma-adaptin-related protein 1; ADP-ribosylation factor binding protein 1; golgi-localized, gamma ear-containing, ARF-binding protein 1; golgi associated, gamma adaptin ear co
• UniProt Protein Name: ADP-ribosylation factor-binding protein GGA1
• Protein Family: ADP-ribosylation factor-binding protein
• UniProt Gene Name: GGA1
• UniProt Entry Name: GGA1_HUMAN

NCBI Description

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GGA1: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Belongs to the GGA protein family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; Golgi apparatus; clathrin adaptor complex; intracellular membrane-bound organelle; membrane; endosome membrane

Molecular Function: protein binding

Biological Process: vesicle-mediated transport; intracellular protein transport; positive regulation of protein catabolic process