Typical Testing Data/Standard Curve (for reference only)
Human superoxide dismutase 2, mitochondrial ELISA Kit | SOD2 elisa kit
Human Superoxide dismutase [Mn], mitochondrial, SOD2 ELISA Kit
Gene Names
SOD2; IPOB; MNSOD; MVCD6
Reactivity
Human
Synonyms
superoxide dismutase 2; mitochondrial; Human Superoxide dismutase [Mn]; SOD2 ELISA Kit; mitochondrial (SOD2) ELISA kit; IPO-B; MNSOD; MVCD6; Mn-SOD; Mn superoxide dismutase; indophenoloxidase B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; SOD2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human SOD2. No significant cross-reactivity or interference between human SOD2 and analogues was observed.
Sequence Length
222
Samples
Serum, plasma, tissue homogenates, cell lysates and cell culture supernates
Assay Type
Quantitative Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
< 3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SOD2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOD2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,722 Da
NCBI Official Full Name
superoxide dismutase
NCBI Official Synonym Full Names
superoxide dismutase 2, mitochondrial
NCBI Official Symbol
SOD2
NCBI Official Synonym Symbols
IPOB; MNSOD; MVCD6
NCBI Protein Information
superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; indophenoloxidase B; Mn superoxide dismutase; mangano-superoxide dismutase; manganese-containing superoxide dismutase
UniProt Protein Name
Superoxide dismutase [Mn], mitochondrial
Protein Family
UniProt Gene Name
SOD2
UniProt Entry Name
SODM_HUMAN
NCBI Description
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.1 PublicationManual assertion based on experiment in:Ref.18
Catalytic activity: 2 superoxide + 2 H+ = O2 + H2O2.
Cofactor: Binds 1 manganese ion per subunit.
Research Articles on SOD2
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Product Notes
The Human SOD2 sod2 (Catalog #AAA706038) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA706038 ELISA Kit recognizes Human SOD2. It is sometimes possible for the material contained within the vial of "superoxide dismutase 2, mitochondrial, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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