Rabbit anti-Human Phosphoinositide 3 Kinase, p110 beta Polyclonal Antibody | anti-PIK3CB antibody

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Cat. Num. AAA620204

• Gene Names: PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Phosphoinositide 3 Kinase; p110 beta; Polyclonal Antibody; p110 beta (DKFZp779K1237; MGC133043; p110beta; Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide; Phosphatidylinositol-4; 5-bisphosphate 3-kinase Catalytic Subunit beta Isoform; Phosphoinositide 3 Kinase Catalytic beta Pol; Anti -Phosphoinositide 3 Kinase; anti-PIK3CB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the internal domain of human PI3 Kinase, p110 beta. Species Sequence Homology: Monkey, canine, Rat and Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-PIK3CB antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.; Dilution: ELISA: 0.5ng/well; Western Blot: 0.05-0.1ug/ml; Immunocytochemistry: 1:100
• Immunogen: Synthetic linear peptide corresponding to the internal domain of PI3 Kinase, p110 beta (KLH).
• Positive Control: HEK-293 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunocytochemistry (ICC)

(Immunocytochemistry: PI3-Kinase, p110b staining of human kidney 2 mm array spot. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. MBS620204 diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is see as a staining to include the thin and distal microtubules.)

Immunocytochemistry (ICC)

(Confocal Immunocytochemistry Analysis: HeLa cells were fixed, permeablized, and stained with MBS620204 (Cy3, red), DAPI (blue, nuclei), and Phalloidin-AlexaFluor®488 (actin, green). Figure on the left has the DAPI filter off.)

Immunohistochemistry (IHC)

(Immunocytochemistry: PI3-Kinase, p110b staining in colorectal carcinoma. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. MBS620204 diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a plasma membrane staining pattern.)

Immunocytochemistry (ICC)

(Immunocytochemistry: PI3-Kinase, p110b staining of human skeletal muscle. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. MBS620204 diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern of cross banding striated muscle fibers.)

Immunoprecipitation (IP)

(Immunoprecipitation: 10ug of MBS620204 was used to immunoprecipitate from 500ug of Jurkat whole cell lysate. The antibody was collected on Protein A beads and eluted with sample buffer. 5uL of Jurkat whole cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 0.5ug of MBS620204. Proteins were visualized using anti-rabbit-HRP conjugate and an ECL system.)

Western Blot (WB)

(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110β (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110β (~110kD).)

Related Product Information for anti-PIK3CB antibody

PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival, signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85kD regulatory subunit and a 110kD catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospholipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the beta:gamma subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

Product Categories/Family for anti-PIK3CB antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 455760
• NCBI GeneID: 5291
• UniProt Accession #: P42338; Q24JU2; D3DNF0; Q68DL0; Q6PJ60; Q9BTS4
• Molecular Weight: 122,762 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase p110 beta isoform
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, catalytic, beta polypeptide
• NCBI Official Symbol: PIK3CB
• NCBI Official Synonym Symbols: PI3K; PIK3C1; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237
• NCBI Protein Information: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3K-beta; OTTHUMP00000216901; OTTHUMP00000216904; PtdIns-3-kinase p110; PI3-kinase subunit beta; PI3-kinase p110 subunit beta; ptdIns-3-kinase subunit beta; ptdIns-3-kinase subunit p110-beta; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
• UniProt Protein Name: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform
• Protein Family: Phosphatidylinositol 4,5-bisphosphate 3-kinase
• UniProt Gene Name: PIK3CB
• UniProt Synonym Gene Names: PIK3C1
• UniProt Entry Name: PK3CB_HUMAN

NCBI Description

Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]

Uniprot Description

PIK3CB: catalytic subunit of phosphoinositide-3-kinase beta. Catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration and cell survival. PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. Expressed ubiquitously.

Protein type: Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 2.7.1.153; Kinase, lipid

Chromosomal Location of Human Ortholog: 3q22.3

Cellular Component: plasma membrane; cytosol; phosphoinositide 3-kinase complex; nucleus

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; insulin receptor substrate binding; 1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; ATP binding

Biological Process: phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; regulation of cell-matrix adhesion; activation of MAPK activity; chemotaxis; signal transduction; T cell receptor signaling pathway; embryonic cleavage; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; autophagy; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway; homophilic cell adhesion; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway