Rabbit 53BP1 Polyclonal Antibody | anti-53BP1 antibody

53BP1 (Phospho-Ser6) Antibody

Cat. Num. AAA9502635

• Gene Names: TP53BP1; TP53; p202; 53BP1; TDRD30; p53BP1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: 53BP1; Polyclonal Antibody; 53BP1 (Phospho-Ser6) Antibody; anti-53BP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: 53BP1 (Phospho-Ser6) Antibody detects endogenous levels of 53BP1 only when phosphorylated at Ser6.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1,975

• Applicable Applications for anti-53BP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:5000
• Target Modification: Phospho
• Modification Sites: H:S6; M:S6; R:S11
• Immunogen: The antiserum was produced against synthesized peptide derived from human 53BP1 around the phosphorylation site of Ser6.
• Immunogen Range: Jan-50
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human heart, using 53BP1 (Phospho-Ser6) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from COS7 cells treated with insulin 0.01U/ML 15', using 53BP1 (Phospho-Ser6) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 213972634
• NCBI GeneID: 7158
• NCBI Accession #: NP_001135451.1
• NCBI GenBank Nucleotide #: NM_001141979.2
• UniProt Accession #: Q12888; Q2M1Z7; Q4LE46; Q5FWZ3; Q7Z3U4; F8VY86
• Molecular Weight: 213 kDa
• NCBI Official Full Name: TP53-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: tumor protein p53 binding protein 1
• NCBI Official Symbol: TP53BP1
• NCBI Official Synonym Symbols: TP53; p202; 53BP1; TDRD30; p53BP1
• NCBI Protein Information: TP53-binding protein 1; tumor suppressor p53-binding protein 1
• UniProt Protein Name: TP53-binding protein 1
• UniProt Gene Name: TP53BP1
• UniProt Synonym Gene Names: p53BP1

NCBI Description

This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Uniprot Description

Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (). In contrast, it is not required for classic NHEJ and V(D)J recombination (). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).