Rat SERP1 ELISA Kit | SERP1 elisa kit

Rat SERP1 (Stress-associated Endoplasmic Reticulum Protein 1) ELISA Kit

Cat. Num. AAA2515943

• Gene Names: SERP1; RAMP4
• Reactivity: Rat
• Synonyms: SERP1; Rat SERP1 (Stress-associated Endoplasmic Reticulum Protein 1) ELISA Kit; SERP1 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinantRatSERP1. No significant cross-reactivity or interference between RatSERP1 and analogues was observed.
• Sequence Length: 66

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SERP1 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatSERP1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SERP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SERP1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SERP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SERP1.You can calculate the concentration of SERP1in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5326499
• NCBI GeneID: 27230
• Molecular Weight: 7,374 Da
• NCBI Official Full Name: SERP1
• NCBI Official Synonym Full Names: stress-associated endoplasmic reticulum protein 1
• NCBI Official Symbol: SERP1
• NCBI Official Synonym Symbols: RAMP4
• NCBI Protein Information: stress-associated endoplasmic reticulum protein 1; ribosome associated membrane protein 4; ribosome-attached membrane protein 4
• UniProt Protein Name: Stress-associated endoplasmic reticulum protein 1
• Protein Family: Stress-associated endoplasmic reticulum protein
• UniProt Gene Name: SERP1
• UniProt Synonym Gene Names: RAMP4
• UniProt Entry Name: SERP1_HUMAN

Uniprot Description

RAMP4: Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins. Belongs to the RAMP4 family.

Protein type: Chaperone; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: endoplasmic reticulum membrane; cytoplasmic microtubule; endoplasmic reticulum; integral to membrane; ribosome

Biological Process: positive regulation of translation; unfolded protein response; glucose metabolic process; multicellular organismal aging; positive regulation of insulin secretion; protein modification process; protein amino acid glycosylation; positive regulation of organ growth; post-embryonic development; positive regulation of growth hormone secretion; protein transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; plasma membrane organization and biogenesis; response to stress; muscle morphogenesis; skeletal development