Polymerase DNA Directed Delta 1 Recombinant Protein | POLd recombinant protein

Recombinant Polymerase DNA Directed Delta 1 (POLd)

Cat. Num. AAA2033314

• Gene Names: POLD1; CDC2; MDPL; POLD; CRCS10
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Polymerase DNA Directed Delta 1; Recombinant Polymerase DNA Directed Delta 1 (POLd); CDC2; POLD1; DNA polymerase subunit delta p125; POLd recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MEEMEAEHRL QEQEEEELQS VLEGVADGQV PPSAIDPRWL RPTPPALDPQ TEPLIFQQLE IDHYVGPAQP VPGGPPPSRG SVPVLRAFGV TDEGFSVCCH IHGFAPYFYT PAPPGFGPEH MGDLQRELNL AISRDSRGGR ELTGPAVLAV ELCSRESMFG YHGHGPSPFL RITVALPRLVAPARRLLEQG IRVAGLGTPS FAPYEANVDF EIRF
• Sequence Length: 1107

• Applicable Applications for POLd recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 53.4kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met41~Phe254
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and GST-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 14250670
• NCBI GeneID: 5424
• Molecular Weight: 123,631 Da
• NCBI Official Full Name: Polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• NCBI Official Synonym Full Names: polymerase (DNA directed), delta 1, catalytic subunit
• NCBI Official Symbol: POLD1
• NCBI Official Synonym Symbols: CDC2; MDPL; POLD; CRCS10
• NCBI Protein Information: DNA polymerase delta catalytic subunit
• UniProt Protein Name: DNA polymerase delta catalytic subunit
• UniProt Gene Name: POLD1
• UniProt Synonym Gene Names: POLD
• UniProt Entry Name: DPOD1_HUMAN

NCBI Description

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

POLD1: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Belongs to the DNA polymerase type-B family.

Protein type: DNA repair, damage; Nucleotide Metabolism - purine; Hydrolase; Transferase; Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA replication

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytoplasm; delta DNA polymerase complex; membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleotide-excision repair complex; nucleus

Molecular Function: 3'-5'-exodeoxyribonuclease activity; 4 iron, 4 sulfur cluster binding; chromatin binding; damaged DNA binding; DNA binding; DNA-directed DNA polymerase activity; metal ion binding; nucleotide binding; protein binding

Biological Process: base-excision repair; base-excision repair, gap-filling; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA repair; DNA replication; DNA replication proofreading; DNA strand elongation during DNA replication; DNA synthesis during DNA repair; fatty acid homeostasis; mismatch repair; mitotic cell cycle; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; response to UV; telomere maintenance; telomere maintenance via recombination; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair

Disease: Colorectal Cancer, Susceptibility To, 10; Mandibular Hypoplasia, Deafness, Progeroid Features, And Lipodystrophy Syndrome