Human Polymerase DNA Directed Delta 1 (POLd) ELISA Kit | POLd elisa kit

Human Polymerase DNA Directed Delta 1 (POLd) ELISA Kit

Cat. Num. AAA2024865

• Gene Names: POLD1; CDC2; MDPL; POLD; CRCS10
• Reactivity: Human
• Synonyms: Polymerase DNA Directed Delta 1 (POLd); Human Polymerase DNA Directed Delta 1 (POLd) ELISA Kit; CDC2; POLD1; DNA polymerase subunit delta p125; POLd elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Polymerase DNA Directed Delta 1 (POLd).; No significant cross-reactivity or interference between Polymerase DNA Directed Delta 1 (POLd) and analogues was observed.
• Sequence Length: 1107

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polymerase DNA Directed Delta 1 (POLd) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polymerase DNA Directed Delta 1 (POLd) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for POLd elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polymerase DNA Directed Delta 1 (POLd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polymerase DNA Directed Delta 1 (POLd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polymerase DNA Directed Delta 1 (POLd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polymerase DNA Directed Delta 1 (POLd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for POLd elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 14250670
• NCBI GeneID: 5424
• Molecular Weight: 123,631 Da
• NCBI Official Full Name: Polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• NCBI Official Synonym Full Names: polymerase (DNA directed), delta 1, catalytic subunit
• NCBI Official Symbol: POLD1
• NCBI Official Synonym Symbols: CDC2; MDPL; POLD; CRCS10
• NCBI Protein Information: DNA polymerase delta catalytic subunit
• UniProt Protein Name: DNA polymerase delta catalytic subunit
• UniProt Gene Name: POLD1
• UniProt Synonym Gene Names: POLD
• UniProt Entry Name: DPOD1_HUMAN

NCBI Description

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

POLD1: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Belongs to the DNA polymerase type-B family.

Protein type: Nucleotide Metabolism - pyrimidine; Hydrolase; DNA repair, damage; Transferase; Nucleotide Metabolism - purine; DNA replication; EC 2.7.7.7

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: delta DNA polymerase complex; nucleoplasm; nucleotide-excision repair complex; membrane; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding; 3'-5'-exodeoxyribonuclease activity; DNA-directed DNA polymerase activity; nucleotide binding; chromatin binding

Biological Process: mismatch repair; DNA strand elongation during DNA replication; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; DNA replication proofreading; base-excision repair; nucleotide-excision repair; fatty acid homeostasis; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA catabolic process, exonucleolytic; DNA replication; telomere maintenance; response to UV

Disease: Mandibular Hypoplasia, Deafness, Progeroid Features, And Lipodystrophy Syndrome; Colorectal Cancer, Susceptibility To, 10