Mouse thrombomodulin ELISA Kit | THBD elisa kit

Mouse Soluable Thrombomodulin, STM ELISA Kit

Cat. Num. AAA704633

• Gene Names: Thbd; TM; CD141; AI385582
• Reactivity: Mouse
• Synonyms: thrombomodulin; Mouse Soluable Thrombomodulin; STM ELISA Kit; Mouse Soluable Thrombomodulin (STM) ELISA KIT; AHUS6; CD141; THRM; TM; CD141 antigen; fetomodulin; THBD elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse THBD. No significant cross-reactivity or interference between Mouse THBD and analogues was observed.
• Sequence Length: 577

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Blood Coagulation
• Protein Biological Process 3: Blood coagulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for THBD elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to sTM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for sTM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain sTM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of sTM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6678339
• NCBI GeneID: 21824
• NCBI Accession #: NP_033404.1
• NCBI GenBank Nucleotide #: NM_009378.3
• UniProt Accession #: P15306
• Molecular Weight: 61,868 Da
• NCBI Official Full Name: thrombomodulin
• NCBI Official Synonym Full Names: thrombomodulin
• NCBI Official Symbol: Thbd
• NCBI Official Synonym Symbols: TM; CD141; AI385582
• NCBI Protein Information: thrombomodulin; fetomodulin; snoRNA MBII-339
• UniProt Protein Name: Thrombomodulin
• Protein Family: Thrombomodulin
• UniProt Gene Name: Thbd
• UniProt Synonym Gene Names: TM
• UniProt Entry Name: TRBM_MOUSE

Uniprot Description

Function: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.