Human apolipoprotein F ELISA Kit | APOF elisa kit

Human Apolipoprotein F, APOF ELISA Kit

Cat. Num. AAA912953

• Gene Names: APOF; LTIP; Apo-F
• Reactivity: Human
• Synonyms: apolipoprotein F; Human Apolipoprotein F; APOF ELISA Kit; Human Apolipoprotein F (APOF) ELISA kit; Apo-F; DKFZp781G18150; LTIP; MGC22520; ; APOF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human APOF. No significant cross-reactivity or interference between human APOF and analogues was observed.
• Sequence Length: 326

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 9.3 8 ng/ml -600ng/ml.
• Sensitivity: The minimum detectable dose of human APOF is typically less than 2.34 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOF elisa kit

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOF. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for APOF. The competitive inhibition reaction is launched between with pre-coated APOF and APOF in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APOF in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4502165
• NCBI GeneID: 319
• NCBI Accession #: NP_001629.1
• NCBI GenBank Nucleotide #: NM_001638.2
• UniProt Accession #: Q13790; Q8TC13
• Molecular Weight: 35,399 Da
• NCBI Official Full Name: apolipoprotein F
• NCBI Official Synonym Full Names: apolipoprotein F
• NCBI Official Symbol: APOF
• NCBI Official Synonym Symbols: LTIP; Apo-F
• NCBI Protein Information: apolipoprotein F; lipid transfer inhibitor protein
• UniProt Protein Name: Apolipoprotein F
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOF
• UniProt Synonym Gene Names: Apo-F; LTIP
• UniProt Entry Name: APOF_HUMAN

NCBI Description

The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol. [provided by RefSeq, Jul 2008]

Uniprot Description

APOF: Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: extracellular region

Molecular Function: lipid transporter activity; cholesterol binding; receptor binding

Biological Process: cholesterol metabolic process; triacylglycerol metabolic process; cholesterol efflux; lipid metabolic process; lipid transport