Typical Testing Data/Standard Curve (for reference only)
General plasminogen total antigen assay ELISA Kit | PLG elisa kit
Mouse plasminogen total antigen assay ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This mouse MCP-1 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse MCP-1. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse MCP-1, if present, will bind and become immobilized by the antibody pre-coated on the wells. A biotin conjugated antibody specific for mouse MCP-1 is added to each well. The biotin conjugated antibody will bind to the mouse MCP-1 on the plate. The microtiter plate wells are thoroughly washed to remove unbound biotin conjugate and other components of the sample. Avidin has a very high affinity to biotin. In order to quantify the amount of mouse MCP-1 present in the sample, a standardized preparation of avidin conjugated horseradish peroxidase (HRP) is added to each well. Avidin-HRP will bind to the biotin on plate during incubation. The wells are thoroughly washed to remove all unbound Avidin-HRP conjugate and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only wells to which mouse MCP-1, Biotin conjugate and Avidin-HRP are attached will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of mouse MCP-1 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus MCP-1 concentration (pg/mL). The concentration of MCP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve!!Background/Introduction: Monocyte chemotactic protein 1(MCP-1), also known as monocyte chemotactic and activating factor(MCAF), lymphocyte-derived chemotactic factor (LDCF) and glioma-derived chemotactic factor (GDF), is a chemotactic cytokine for monocytes. It is a CC chemokine with two adjacent cystines at its NH2 terminus. MCP1 is produced by a variety of cell types, including monocytes, lymphocytes, fibroblasts, endothelial cells, epithelial cells and smooth muscle cells. MCP-1 is up-regulated in response to infectious agents, oxidative radicals and pro-inflammatory mediators released by host cells. The major physiological function of MCP-1 is to mediate host defense. MCP-1 acts as a chemoattractant to recruit monocytes to the infected or injured area and activates the cells to secret cytokines and superoxide, enhances phagocytosis and antigen presentation by macrophages. The expression patterns of MCP-1 and IL-8 are similar. Both cytokines will increase upon stimulation with LPS and inflammatory cytokines such as IL-1, TNF-alpha, and IFN-gamma. In mouse fibroblasts, platelet derived growth factor (PDGF) is a strong inducer of MCP-1 mRNA expression but failed to induce IL-8 mRNA, suggesting different regulatory mechanisms. In addition to being chemotactic for monocytes, MCP-1 also activates mouse monocytes to become cytostatic for several mouse tumour cell lines, release lysosomal enzymes, and generate superoxide. Elevated MCP-1 levels are associated with autoimmune disease, allergic inflammation, atherosclerosis, glomerulonephritis, granuloma formation. This ELISA kit provides a tool for studying MCP-1 expression and its relationship with various diseases in animal model.
NCBI and Uniprot Product Information
Uniprot Description
Plasminogen: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Defects in PLG are the cause of plasminogen deficiency (PLGD). PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. Belongs to the peptidase S1 family. Plasminogen subfamily.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Protease; EC 3.4.21.7
Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; extrinsic to external side of plasma membrane; intracellular membrane-bound organelle; cell; extracellular region; plasma membrane
Molecular Function: peptidase activity; protein domain specific binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; endopeptidase activity; apolipoprotein binding; catalytic activity; receptor binding
Biological Process: tissue remodeling; proteolysis; muscle maintenance; myoblast differentiation; extracellular matrix disassembly; negative regulation of angiogenesis; fibrinolysis; negative regulation of fibrinolysis; proteolysis involved in cellular protein catabolic process; hemostasis; tissue regeneration; positive regulation of fibrinolysis; blood coagulation
Research Articles on PLG
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Product Notes
The General PLG plg (Catalog #AAA135494) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135494 ELISA Kit recognizes General PLG. It is sometimes possible for the material contained within the vial of "plasminogen total antigen assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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