Human apolipoprotein C-II ELISA Kit | APOC2 elisa kit

Human Apolipoprotein C2, apo-C2 ELISA Kit

Cat. Num. AAA700826

• Gene Names: APOC2; APO-CII; APOC-II
• Reactivity: Human
• Synonyms: apolipoprotein C-II; Human Apolipoprotein C2; apo-C2 ELISA Kit; MGC75082; ; APOC2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human apo-C2. No significant cross-reactivity or interference between human apo-C2 and analogues was observed.
• Sequence Length: 101

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 31.2 ng/ml -2000 ng/ml.
• Sensitivity: The minimum detectable dose of human apo-C2 is typically less than 15.6 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human apo-C2 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOC2 elisa kit

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with apo-C2. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for apo-C2. The competitive inhibition reaction is launched between with pre-coated apo-C2 and apo-C2 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of apo-C2 in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 32130518
• NCBI GeneID: 344
• NCBI Accession #: NP_000474.2
• NCBI GenBank Nucleotide #: NM_000483.4
• UniProt Accession #: P02655; Q9BS39; Q9UDE3; Q9UNK3; C0JYY4
• Molecular Weight: 11,284 Da
• NCBI Official Full Name: apolipoprotein C-II
• NCBI Official Synonym Full Names: apolipoprotein C-II
• NCBI Official Symbol: APOC2
• NCBI Official Synonym Symbols: APO-CII; APOC-II
• NCBI Protein Information: apolipoprotein C-II; apolipoprotein C2
• UniProt Protein Name: Apolipoprotein C-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC2
• UniProt Synonym Gene Names: APC2; Apo-CII; ApoC-II
• UniProt Entry Name: APOC2_HUMAN

NCBI Description

This gene encodes a lipid-binding protein belonging to the apolipoprotein gene family. The protein is secreted in plasma where it is a component of very low density lipoprotein. This protein activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells. Mutations in this gene cause hyperlipoproteinemia type IB, characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. This gene is present in a cluster with other related apolipoprotein genes on chromosome 19. Naturally occurring read-through transcription exists between this gene and the neighboring upstream apolipoprotein C-IV (APOC4) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

Function: Component of the very low density lipoprotein (VLDL) fraction in plasma, and is an activator of several triacylglycerol lipases. The association of APOC2 with plasma chylomicrons, VLDL, and HDL is reversible, a function of the secretion and catabolism of triglyceride-rich lipoproteins, and changes rapidly.