Sheep Phospholipase C Gamma 1 ELISA Kit | PLCg1 elisa kit

Sheep Phospholipase C Gamma 1 ELISA Kit

Cat. Num. AAA741230

• Reactivity: Sheep
• Synonyms: Phospholipase C Gamma 1; Sheep Phospholipase C Gamma 1 ELISA Kit; PLCg1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This PLCg1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PLCg1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLCg1 elisa kit

Principle of the assay: PLCg1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLCg1 antibody and an PLCg1-HRP conjugate. The assay sample and buffer are incubated together with PLCg1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLCg1 concentration since PLCg1 from samples and PLCg1-HRP conjugate compete for the anti-PLCg1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PLCg1 from the sample, fewer sites are left to bind PLCg1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLCg1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PLCg1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 3336979
• UniProt Accession #: P19174; Q2V575; B7ZLY7; B9EGH4; E1P5W4
• Molecular Weight: 148,660 Da
• UniProt Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
• Protein Family: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
• UniProt Gene Name: PLCG1
• UniProt Synonym Gene Names: PLC1; PLC-II; PLC-gamma-1
• UniProt Entry Name: PLCG1_HUMAN

Uniprot Description

PLCG1: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.

Protein type: EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate; Phospholipase; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: signalosome; ruffle; cell projection; lamellipodium; cytoplasm; plasma membrane; intercellular junction; cytosol

Molecular Function: protein binding; glutamate receptor binding; neurotrophin TRKA receptor binding; receptor signaling protein activity; calcium ion binding; receptor tyrosine kinase binding; phospholipase C activity; protein kinase binding; phosphoinositide phospholipase C activity

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; cell migration; fibroblast growth factor receptor signaling pathway; inositol phosphate metabolic process; activation of MAPKK activity; nerve growth factor receptor signaling pathway; viral reproduction; in utero embryonic development; phospholipid catabolic process; cytokine and chemokine mediated signaling pathway; positive regulation of blood vessel endothelial cell migration; calcium-mediated signaling; signal transduction; T cell receptor signaling pathway; positive regulation of angiogenesis; phospholipase C activation; innate immune response; positive regulation of release of sequestered calcium ion into cytosol; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration