Human Phospholipase C Gamma 1 ELISA Kit | PLCg1 elisa kit

Human Phospholipase C Gamma 1 ELISA Kit

Cat. Num. AAA726751

• Reactivity: Human
• Synonyms: Phospholipase C Gamma 1; Human Phospholipase C Gamma 1 ELISA Kit; PLCg1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PLCg1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PLCg1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLCg1 elisa kit

Principle of the Assay: PLCg1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PLCg1. Standards or samples are then added to the microtiter plate wells and PLCg1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PLCg1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PLCg1 are added to each well to "sandwich" the PLCg1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PLCg1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLCg1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PLCg1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 3336979
• UniProt Accession #: P19174; Q2V575; B7ZLY7; B9EGH4; E1P5W4
• Molecular Weight: 148,660 Da
• UniProt Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
• Protein Family: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
• UniProt Gene Name: PLCG1
• UniProt Synonym Gene Names: PLC1; PLC-II; PLC-gamma-1
• UniProt Entry Name: PLCG1_HUMAN

Uniprot Description

PLCG1: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.

Protein type: EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; Phospholipase

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: signalosome; ruffle; cell projection; lamellipodium; cytoplasm; plasma membrane; intercellular junction; cytosol

Molecular Function: protein binding; glutamate receptor binding; neurotrophin TRKA receptor binding; receptor signaling protein activity; calcium ion binding; receptor tyrosine kinase binding; protein kinase binding; phospholipase C activity; phosphoinositide phospholipase C activity

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; cell migration; fibroblast growth factor receptor signaling pathway; inositol phosphate metabolic process; activation of MAPKK activity; nerve growth factor receptor signaling pathway; viral reproduction; phospholipid catabolic process; in utero embryonic development; cytokine and chemokine mediated signaling pathway; positive regulation of blood vessel endothelial cell migration; calcium-mediated signaling; signal transduction; T cell receptor signaling pathway; positive regulation of angiogenesis; phospholipase C activation; innate immune response; positive regulation of release of sequestered calcium ion into cytosol; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration