Goat Phospholipase C Gamma 1 ELISA Kit | PLCg1 elisa kit

Goat Phospholipase C Gamma 1 ELISA Kit

Cat. Num. AAA740976

• Gene Names: PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
• Reactivity: Goat
• Synonyms: Phospholipase C Gamma 1; Goat Phospholipase C Gamma 1 ELISA Kit; PLCg1 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This PLCg1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat PLCg1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLCg1 elisa kit

Principle of the Assay: PLCg1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLCg1 antibody and an PLCg1-HRP conjugate. The assay sample and buffer are incubated together with PLCg1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLCg1 concentration since PLCg1 from samples and PLCg1-HRP conjugate compete for the anti-PLCg1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PLCg1 from the sample, fewer sites are left to bind PLCg1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLCg1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PLCg1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 3336979
• NCBI GeneID: 5335
• UniProt Accession #: P19174; Q2V575; B7ZLY7; B9EGH4; E1P5W4; Q4LE43; Q6LC80; Q9UFY1
• Molecular Weight: 148,532 Da
• NCBI Official Full Name: phospholipase C, gamma 1
• NCBI Official Synonym Full Names: phospholipase C, gamma 1
• NCBI Official Symbol: PLCG1
• NCBI Official Synonym Symbols: PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
• NCBI Protein Information: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; PLC-gamma-1; phospholipase C-II; phospholipase C-148; phosphoinositidase C; phospholipase C-gamma-1; inositoltrisphosphohydrolase; phosphoinositide phospholipase C; phosphatidylinositol phospholipase C; triphosphoinositide phosphodiesterase; phosphoinositide phospholipase C-gamma-1; monophosphatidylinositol phosphodiesterase; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; phospholipase C, gamma 1 (formerly subtype 148); 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
• UniProt Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1
• Protein Family: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
• UniProt Gene Name: PLCG1
• UniProt Synonym Gene Names: PLC1; PLC-II; PLC-gamma-1
• UniProt Entry Name: PLCG1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PLCG1: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.

Protein type: Motility/polarity/chemotaxis; Phospholipase; Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: signalosome; ruffle; cell projection; lamellipodium; cytoplasm; plasma membrane; intercellular junction; cytosol

Molecular Function: protein binding; glutamate receptor binding; neurotrophin TRKA receptor binding; receptor signaling protein activity; calcium ion binding; receptor tyrosine kinase binding; protein kinase binding; phospholipase C activity; phosphoinositide phospholipase C activity

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; cell migration; fibroblast growth factor receptor signaling pathway; inositol phosphate metabolic process; nerve growth factor receptor signaling pathway; viral reproduction; activation of MAPKK activity; phospholipid catabolic process; in utero embryonic development; cytokine and chemokine mediated signaling pathway; positive regulation of blood vessel endothelial cell migration; calcium-mediated signaling; signal transduction; T cell receptor signaling pathway; positive regulation of angiogenesis; phospholipase C activation; innate immune response; positive regulation of release of sequestered calcium ion into cytosol; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration