Rabbit ILK Polyclonal Antibody | anti-ILK antibody

ILK Polyclonal Antibody

Cat. Num. AAA125125

• Gene Names: ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: ILK; Polyclonal Antibody; ILK Polyclonal Antibody; DKFZp686F1765; P59; ILK1; anti-ILK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 452

• Applicable Applications for anti-ILK antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:1000; IHC: 1:50 - 1:100
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human ILK
• Calculated Molecular Weight: 59kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ILK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using ILK Antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using ILK Antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using ILK Antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using ILK Antibody at dilution of 1:200 (40x lens).)

Related Product Information for anti-ILK antibody

Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2).

Product Categories/Family for anti-ILK antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 9973397
• NCBI GeneID: 3611
• UniProt Accession #: Q13418; P57043; Q68DZ3; B7Z1I0; B7Z418; D3DQU0
• Molecular Weight: 452
• NCBI Official Full Name: Integrin-linked protein kinase
• NCBI Official Synonym Full Names: integrin-linked kinase
• NCBI Official Symbol: ILK
• NCBI Official Synonym Symbols: P59; ILK-1; ILK-2; p59ILK; HEL-S-28
• NCBI Protein Information: integrin-linked protein kinase; integrin-linked kinase-2; epididymis secretory protein Li 28; 59 kDa serine/threonine-protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• Protein Family: Integrin-linked protein kinase
• UniProt Gene Name: ILK
• UniProt Synonym Gene Names: ILK1; ILK2
• UniProt Entry Name: ILK_HUMAN

NCBI Description

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, TKL; TKL group; MLK family; ILK subfamily

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: focal adhesion; protein complex; costamere; terminal button; intercellular junction; cytosol; nucleoplasm; sarcomere; membrane; cell soma; lamellipodium; cytoplasm; plasma membrane; stress fiber; dendritic shaft; cell junction

Molecular Function: integrin binding; protein serine/threonine kinase activity; signal transducer activity; protein binding; protein kinase binding; SH3 domain binding; ATP binding

Biological Process: negative regulation of smooth muscle cell proliferation; protein heterooligomerization; cell-matrix adhesion; positive regulation of transcription, DNA-dependent; positive regulation of axon extension; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; myelin formation; positive regulation of MAP kinase activity; positive regulation of cell proliferation; protein kinase B signaling cascade; fibril organization and biogenesis; negative regulation of neuron apoptosis; positive regulation of cell-matrix adhesion; cell cycle arrest; positive regulation of BMP signaling pathway; integrin-mediated signaling pathway; cell aging; positive regulation of dendrite morphogenesis; positive regulation of myoblast differentiation; myelination in the peripheral nervous system; establishment and/or maintenance of epithelial cell polarity; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; cell proliferation; peptidyl-serine phosphorylation; ureteric bud branching; regulation of actin cytoskeleton organization and biogenesis; negative regulation of protein kinase activity; nerve development; neurite morphogenesis; positive regulation of phosphorylation; positive regulation of cell migration