Rabbit anti-Human Acetylcholinesterase (AchE) Polyclonal Antibody | anti-AchE antibody

Polyclonal Antibody to Acetylcholinesterase (AchE)

Cat. Num. AAA2004480

• Gene Names: ACHE; YT; ACEE; ARACHE; N-ACHE
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Acetylcholinesterase (AchE); Polyclonal Antibody; Polyclonal Antibody to Acetylcholinesterase (AchE); anti-AchE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against AchE. It has been selected for its ability to recognize AchE in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF- FSKD NESLISRAEF LAGVRVGVPQ VSDLAAEAVV LHYTDWLHPE DPARLREALS DVVGDHNVVC PVAQLAGRLA AQGARVYAYV FEHRASTLSW PLWMGVPHGY EIEFIFGIPL DPSRNYTAEE KIFAQRLMRY WANFARTGDP NEPRDPKAPQ WPPYTAGAQQ YVSLDLRPLE VRRGLRAQAC AFWNRFLPKL LSAT
• Sequence Length: 72

• Applicable Applications for anti-AchE antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant AchE (Phe377~Thr574) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2055255
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant AchE, Human.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

• NCBI GI #: 4557239
• NCBI GeneID: 43
• NCBI Accession #: NP_000656.1
• NCBI GenBank Nucleotide #: NM_000665.4
• UniProt Accession #: P22303; Q16169; Q29S23; Q2M324; Q504V3; Q53F46; Q86TM9; Q86YX9; Q9BXP7; A4D2E2; B7ZKZ0; D6W5X7
• Molecular Weight: 58,352 Da
• NCBI Official Full Name: acetylcholinesterase isoform E4-E6
• NCBI Official Synonym Full Names: acetylcholinesterase (Cartwright blood group)
• NCBI Official Symbol: ACHE
• NCBI Official Synonym Symbols: YT; ACEE; ARACHE; N-ACHE
• NCBI Protein Information: acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: ACHE
• UniProt Synonym Gene Names: AChE

NCBI Description

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Uniprot Description

AChE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.7; Hydrolase; Lipid Metabolism - glycerophospholipid; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: basal lamina; cell junction; cell surface; extracellular region; extracellular space; Golgi apparatus; membrane; neuromuscular junction; nucleus; perinuclear region of cytoplasm; plasma membrane; synapse

Molecular Function: acetylcholine binding; acetylcholinesterase activity; amyloid-beta binding; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; protein binding; protein homodimerization activity; protein self-association; serine hydrolase activity

Biological Process: acetylcholine catabolic process; acetylcholine catabolic process in synaptic cleft; amyloid precursor protein metabolic process; cell adhesion; cell proliferation; DNA replication; muscle organ development; negative regulation of synaptic transmission, cholinergic; nervous system development; neurotransmitter biosynthetic process; neurotransmitter receptor biosynthetic process; osteoblast development; phosphatidylcholine biosynthetic process; positive regulation of protein secretion; protein tetramerization; receptor internalization; regulation of receptor recycling; response to wounding; retina development in camera-type eye; synaptogenesis

Disease: Yt Blood Group Antigen