Rat Pyruvate kinase isozymes M1/M2 ELISA Kit | Pkm2 elisa kit

Rat Pyruvate kinase isozymes M1/M2 ELISA Kit

Cat. Num. AAA2883448

• Gene Names: Pkm; Pk3; Pkm2; PKM12
• Reactivity: Rat
• Synonyms: Pyruvate kinase isozymes M1/M2; Rat Pyruvate kinase isozymes M1/M2 ELISA Kit; Pyruvate kinase muscle isozyme; Pkm2; Pykm; 2.7.1.40; Pkm2 elisa kit

• Reactivity: Rat
• Sequence Length: 531

• Assay Type: Sandwich
• Detection Range: 1.25-80 U/mL
• Sensitivity: 0.625 U/mL
• Intra-Assay CV: <=5.1%
• Inter-Assay CV: <=7.9%
• Recovery: 89%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 16757994
• NCBI GeneID: 25630
• NCBI Accession #: NP_445749.1
• NCBI GenBank Nucleotide #: NM_053297.2
• UniProt Accession #: P11980; P11981
• Molecular Weight: 57,781 Da
• NCBI Official Full Name: pyruvate kinase PKM
• NCBI Official Synonym Full Names: pyruvate kinase, muscle
• NCBI Official Symbol: Pkm
• NCBI Official Synonym Symbols: Pk3; Pkm2; PKM12
• NCBI Protein Information: pyruvate kinase PKM
• UniProt Protein Name: Pyruvate kinase PKM
• UniProt Gene Name: Pkm
• UniProt Synonym Gene Names: Pkm2; Pykm
• UniProt Entry Name: KPYM_RAT

NCBI Description

M1 and M2 isozymes of pyruvate kinase; catalyzes the conversion of ATP and pyruvate to ADP and phosphoenolpyruvate in glycolysis [RGD, Feb 2006]

Uniprot Description

PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.

Protein type: Carbohydrate Metabolism - pyruvate; Nucleotide Metabolism - purine; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.1.40

Cellular Component: cilium; cytoplasm; extracellular matrix; mitochondrion; myelin sheath; nucleus; plasma membrane; vesicle

Molecular Function: ADP binding; ATP binding; kinase activity; magnesium ion binding; potassium ion binding; protein binding; pyruvate kinase activity

Biological Process: ATP biosynthetic process; glucose metabolic process; glycolysis; liver development; organ regeneration; programmed cell death; pyruvate biosynthetic process; response to gravity; response to hypoxia; response to insulin stimulus; response to muscle inactivity; response to nutrient; response to organic substance; skeletal muscle regeneration