Rabbit anti-Human BBC3 Polyclonal Antibody | anti-BBC3 antibody

BBC3 Antibody (C-Term)

Cat. Num. AAA9216342

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Human
• Applications: Western Blot
• Synonyms: BBC3; Polyclonal Antibody; BBC3 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis; PUMA; anti-BBC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This BBC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 158-192 amino acids from human BBC3.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 261

• Applicable Applications for anti-BBC3 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-BBC3 Antibody (C-Term) at 1:2000 dilution + A549 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-BBC3 antibody

Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity.

References

Nakano K.,et al.Mol. Cell 7:683-694(2001). Grimwood J.,et al.Nature 428:529-535(2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 187829730
• NCBI GeneID: 27113
• NCBI Accession #: NP_001120712.1
• NCBI GenBank Nucleotide #: NM_001127240.2
• UniProt Accession #: Q96PG8; Q9BXH1
• Molecular Weight: 26498
• NCBI Official Full Name: bcl-2-binding component 3 isoform 1
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3
• UniProt Protein Name: Bcl-2-binding component 3
• Protein Family: Bcl-2-binding component
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3B_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

BBC3B: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity. Induced by DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. {ECO:0000269|PubMed:11463392}. Isoform 3 does not interact with BCL2. 4 isoforms of the human protein are produced by alternative splicing

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; programmed cell death; determination of adult life span; response to DNA damage stimulus; negative regulation of growth