SDS-PAGE
STK16 recombinant protein
STK16 recombinant protein
SDS-PAGE
SDS-PAGE
Scientific Background: STK16 is serine/threonine kinase 16 which constitute the catalytic subunit of a multisubunit kinase. STK16 is ubiquitously expressed at very low levels and expression of an STK16 fusion protein showed that STK16 is localized with Golgi and Golgi-derived vesicles. The overexpression of STK16 leads to disorganization of the Golgi apparatus into vesicular structures and STK16 is highlyinvolved in the secretory pathway (1). STK16 is involved in the transforming growth factor (TGF)-beta signalling pathway (2).
2. Ohta, S. et.al: A novel transcriptional factor with Ser/Thr kinase activity involved in the transforming growth factor (TGF)-beta signalling pathway. Biochem. J. 350: 395-404, 2000.
NCBI and Uniprot Product Information
Uniprot Description
Function: Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins. Ref.2
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure: Monomer. Interacts with DRG1 (via its N-terminal); the interaction phosphorylates DRG1. Ref.10
Subcellular location: Cytoplasm › perinuclear region. Membrane; Lipid-anchor
By similarity. Note: Associates with Golgi and Golgi-derived vesicles
By similarity.
Tissue specificity: Ubiquitously expressed at very low levels. Ref.2
Post-translational modification: Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198. Ref.2 Ref.10It is uncertain whether palmitoylation is on Cys-6 and/or Cys-8. Ref.2
Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 protein kinase domain.
Sequence caution: The sequence AAV38392.1 differs from that shown. Reason: Frameshift at position 305. The sequence CAA06700.1 differs from that shown. Reason: Frameshift at position 305.
Research Articles on STK16
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Product Notes
The STK16 stk16 (Catalog #AAA515156) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human STK16 was expressed by baculovirus in Sf9 insect cells. AAA Biotech's STK16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the STK16 stk16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK16, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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