Mouse phosphoinositide-3-kinase, catalytic, alpha polypeptide ELISA Kit | PIK3CA elisa kit

Mouse Phosphotylinosital 3 kinase, PI3K ELISA Kit

Cat. Num. AAA703575

• Gene Names: Pik3ca; p110; caPI3K; p110alpha; 6330412C24Rik
• Reactivity: Mouse
• Synonyms: phosphoinositide-3-kinase; catalytic; alpha polypeptide; Mouse Phosphotylinosital 3 kinase; PI3K ELISA Kit; MGC142161; MGC142163; PI3K; p110-alpha; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; phosphatidylinositol 3-kinase; 110-KD; alpha; alpha pol; PIK3CA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PI3K. No significant cross-reactivity or interference between mouse PI3K and analogues was observed.
• Sequence Length: 1068

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 23.5 pg/ml -1500 pg/ml.
• Sensitivity: The minimum detectable dose of mouse PI3K is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PIK3CA elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PI3K has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PI3K present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI3K is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI3K bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 254540080
• NCBI GeneID: 18706
• NCBI Accession #: NP_032865.2
• NCBI GenBank Nucleotide #: NM_008839.2
• UniProt Accession #: P42337; Q0VGQ5
• Molecular Weight: 124,412 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
• NCBI Official Symbol: Pik3ca
• NCBI Official Synonym Symbols: p110; caPI3K; p110alpha; 6330412C24Rik
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3Kalpha; PI3K-alpha; PI3-kinase subunit alpha; ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein kinase PIK3CA; phosphoinositide-3-kinase catalytic alpha polypeptide; phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• Protein Family: Phosphatidylinositol 4,5-bisphosphate 3-kinase
• UniProt Gene Name: Pik3ca
• UniProt Synonym Gene Names: PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha
• UniProt Entry Name: PK3CA_MOUSE

Uniprot Description

Function: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.6 PublicationsManual assertion based on experiment in:Ref.5

Catalytic activity: ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.