Human growth differentiation factor 10 ELISA Kit | GDF10 elisa kit

Human Bone morphogenetic protein 3B, GDF10 ELISA Kit

Cat. Num. AAA945713

• Gene Names: GDF10; BMP3B; BMP-3b
• Reactivity: Human
• Synonyms: growth differentiation factor 10; Human Bone morphogenetic protein 3B; GDF10 ELISA Kit; Human Bone morphogenetic protein 3B (GDF10) ELISA kit; BMP-3b; BMP3B; bone morphogenetic protein 3B; GDF10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human GDF10. No significant cross-reactivity or interference between human GDF10 and analogues was observed.
• Sequence Length: 478

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 23.44 pg/ml-1500 pg/ml.
• Sensitivity: The minimum detectable dose of human GDF10 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF10 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GDF10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDF10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GDF10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDF10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4826740
• NCBI GeneID: 2662
• NCBI Accession #: NP_004953.1
• NCBI GenBank Nucleotide #: NM_004962.3
• UniProt Accession #: P55107; Q5VSQ8; Q9UCX6
• Molecular Weight: 53,122 Da
• NCBI Official Full Name: bone morphogenetic protein 3B
• NCBI Official Synonym Full Names: growth differentiation factor 10
• NCBI Official Symbol: GDF10
• NCBI Official Synonym Symbols: BMP3B; BMP-3b
• NCBI Protein Information: bone morphogenetic protein 3B; BIP; GDF-10; bone-inducing protein
• UniProt Protein Name: Bone morphogenetic protein 3B
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF10
• UniProt Synonym Gene Names: BMP3B; BMP-3B; BIP; GDF-10
• UniProt Entry Name: BMP3B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that the protein encoded by this gene plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF10: is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice suggest that plays a role in skeletal morphogenesis. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; fat cell differentiation; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; skeletal development; cell development; growth