Human lysine (K) -specific demethylase 5A ELISA Kit | KDM5A elisa kit

Human Lysine-specific demethylase 5A, KDM5A ELISA Kit

Cat. Num. AAA938758

• Gene Names: KDM5A; RBP2; RBBP2; RBBP-2
• Reactivity: Human
• Synonyms: lysine (K) -specific demethylase 5A; Human Lysine-specific demethylase 5A; KDM5A ELISA Kit; Human Lysine-specific demethylase 5A (KDM5A) ELISA kit; JARID1A; RBBP2; RBP2; Jumonji; AT rich interactive domain 1A (RBBP2-like) ; AT rich interactive domain 1A (RBP2-like) ; retinoblastoma binding protein 2; retinoblastoma-binding protein 2; KDM5A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human KDM5A. No significant cross-reactivity or interference between human KDM5A and analogues was observed.
• Sequence Length: 1,690

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 23.5 pg/ml -1500 pg/ml.
• Sensitivity: The minimum detectable dose of human KDM5A is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KDM5A elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KDM5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KDM5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDM5A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDM5A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 110618244
• NCBI GeneID: 5927
• NCBI Accession #: NP_001036068.1
• NCBI GenBank Nucleotide #: NM_001042603.2
• UniProt Accession #: P29375; Q4LE72; Q86XZ1; A8MV76
• Molecular Weight: 192,095 Da
• NCBI Official Full Name: lysine-specific demethylase 5A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 5A
• NCBI Official Symbol: KDM5A
• NCBI Official Synonym Symbols: RBP2; RBBP2; RBBP-2
• NCBI Protein Information: lysine-specific demethylase 5A; histone demethylase JARID1A; retinoblastoma binding protein 2; jumonji/ARID domain-containing protein 1A; Jumonji, AT rich interactive domain 1A (RBP2-like)
• UniProt Protein Name: Lysine-specific demethylase 5A
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM5A
• UniProt Synonym Gene Names: JARID1A; RBBP2; RBP2; RBBP-2
• UniProt Entry Name: KDM5A_HUMAN

NCBI Description

This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]

Uniprot Description

JARID1A: Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Interacts with SUZ12; the interaction is direct. Interacts with the viral protein-binding domain of RB1. Interacts with ESR1, MYC, MYCN and LMO2. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 1.14.11.-; Nucleolus; Oxidoreductase; Transcription factor

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription coactivator activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; multicellular organismal development; positive regulation of transcription, DNA-dependent; chromatin modification; circadian regulation of gene expression