Human protein disulfide isomerase family A, member 4 ELISA Kit | PDIA4 elisa kit

Human Protein disulfide-isomerase A4, PDIA4 ELISA Kit

Cat. Num. AAA915359

• Gene Names: PDIA4; ERP70; ERP72; ERp-72
• Reactivity: Human
• Synonyms: protein disulfide isomerase family A; member 4; Human Protein disulfide-isomerase A4; PDIA4 ELISA Kit; Human Protein disulfide-isomerase A4 (PDIA4) ELISA kit; ERP70; ERP72; protein disulfide isomerase A4; protein disulfide isomerase related protein (calcium-binding protein; intestinal-related) ; protein disulfide isomerase-associated 4; PDIA4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PDIA4. No significant cross-reactivity or interference between human PDIA4 and analogues was observed.
• Sequence Length: 645

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 23.5 pg/ml -1500 pg/ml.
• Sensitivity: The minimum detectable dose of human PDIA4 is typically less than 5.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDIA4 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDIA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDIA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4758304
• NCBI GeneID: 9601
• NCBI Accession #: NP_004902.1
• NCBI GenBank Nucleotide #: NM_004911.4
• UniProt Accession #: P13667; Q549T6; A8K4K6
• Molecular Weight: 72,932 Da
• NCBI Official Full Name: protein disulfide-isomerase A4
• NCBI Official Synonym Full Names: protein disulfide isomerase family A, member 4
• NCBI Official Symbol: PDIA4
• NCBI Official Synonym Symbols: ERP70; ERP72; ERp-72
• NCBI Protein Information: protein disulfide-isomerase A4; ER protein 70; ER protein 72; protein disulfide isomerase-associated 4; endoplasmic reticulum resident protein 70; endoplasmic reticulum resident protein 72; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related)
• UniProt Protein Name: Protein disulfide-isomerase A4
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA4
• UniProt Synonym Gene Names: ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72
• UniProt Entry Name: PDIA4_HUMAN

Uniprot Description

PDIA4: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the protein disulfide isomerase family.

Protein type: EC 5.3.4.1; Isomerase; Endoplasmic reticulum; Chaperone

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: protein folding; cell redox homeostasis; protein secretion