Glutamate Pyruvate Transaminase Active Protein | GPT active protein

Glutamate Pyruvate Transam Ag

Cat. Num. AAA5303584

• Gene Names: GPT; AAT1; ALT1; GPT1
• Synonyms: Glutamate Pyruvate Transaminase; Glutamate Pyruvate Transam Ag; Alanine aminotransferase protein; GPT active protein

• Host: Pig
• Form/Format: Lyophilized from Tris-chloride buffer, pH 7.0 with sodium chloride, EDTA, DTT, alpha-ketoglutarate, and pyridoxal-5-phosphate.
• Sequence Length: 496

• Source Note: Porcine Heart
• Protein Type: Native
• Activity: 90 units/mg
• Protein: 0.99mg protein/mg (A280 - Q0033); 0.50mg protein/mg (Coomassie - Q0033)
• Specific Activity: 90U/mg protein (A280); 180U/mg protein (Coomassie)
• Contaminants: CPK: 0.05%; GLDH: <0.01%; LDH: <0.01%; SGOT/AST: <0.1%; Protease: <0.1 U/mg (<0.001 OD/8 minutes)
• Biohazard: User standard laboratory precautions when handling this material.
• Preparation and Storage: 4 degree C shipping. Upon receipt, store at -20 degree C.

Related Product Information for GPT active protein

Purified native Porcine Glutamate Pyruvate Transaminase protein

Product Categories/Family for GPT active protein

Proteases; Inhibitors; & Enzymes; Native Protein

NCBI and Uniprot Product Information

• NCBI GI #: 1763096
• NCBI GeneID: 2875
• Molecular Weight: 100 kDa
• NCBI Official Full Name: glutamate pyruvate transaminase
• NCBI Official Synonym Full Names: glutamic-pyruvate transaminase (alanine aminotransferase)
• NCBI Official Symbol: GPT
• NCBI Official Synonym Symbols: AAT1; ALT1; GPT1
• NCBI Protein Information: alanine aminotransferase 1
• UniProt Protein Name: Alanine aminotransferase 1
• Protein Family: Alanine aminotransferase
• UniProt Gene Name: GPT
• UniProt Synonym Gene Names: AAT1; GPT1; ALT1; GPT 1
• UniProt Entry Name: ALAT1_HUMAN

NCBI Description

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

Uniprot Description

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine catabolic process