Rabbit anti-Human USP4 Polyclonal Antibody | anti-USP4 antibody

USP4, CT (Ubiquitin Carboxyl-terminal Hydrolase 4, Ubiquitin Thiolesterase 4, Ubiquitin Specific-processing Protease 4, Deubiquitinating Enzyme 4, Ubiquitous Nuclear Protein Homolog, UNP, UNPH)

Cat. Num. AAA629682

• Gene Names: USP4; UNP; Unph; MGC149848; MGC149849
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: USP4; Polyclonal Antibody; CT (Ubiquitin Carboxyl-terminal Hydrolase 4; Ubiquitin Thiolesterase 4; Ubiquitin Specific-processing Protease 4; Deubiquitinating Enzyme 4; Ubiquitous Nuclear Protein Homolog; UNP; UNPH); Anti -USP4; anti-USP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP4.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-USP4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human USP4 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-USP4 C-term Pab is used in Western blot to detect USP4 in USP4-transfected HeLa cell lysate. Transfection data is kindly provided by Dr. B. Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-USP4 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Product Categories/Family for anti-USP4 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 40795665
• NCBI GeneID: 7375
• NCBI Accession #: NP_003354.2
• NCBI GenBank Nucleotide #: NM_003363.3
• UniProt Accession #: Q08AK7; Q13107; Q53FJ3; Q53H56
• Molecular Weight: 108,523 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 4 isoform a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 4 (proto-oncogene)
• NCBI Official Symbol: USP4
• NCBI Official Synonym Symbols: UNP; Unph; MGC149848; MGC149849
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 4; OTTHUMP00000210715; OTTHUMP00000210716; OTTHUMP00000210717; ubiquitin thioesterase 4; deubiquitinating enzyme 4; ubiquitin thiolesterase 4; ubiquitous nuclear protein homolog; ubiquitin carboxyl-terminal esterase 4; ubiquitin-specific processing protease 4; ubiquitin-specific-processing protease 4; ubiquitin specific protease 4 (proto-oncogene)
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP4
• UniProt Entry Name: Q08AK7_HUMAN

NCBI Description

The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

USP4: Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER. Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2. Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung. Expressed in the hippocampal neurons. Belongs to the peptidase C19 family. USP4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Ubiquitin conjugating system; Protease; Oncoprotein

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: lysosome; cytoplasm; plasma membrane; nucleus

Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; metal ion binding; ubiquitin-specific protease activity; adenosine receptor binding

Biological Process: assembly of spliceosomal tri-snRNP; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of protein stability; negative regulation of protein ubiquitination