Sheep Glutamic Pyruvic Transaminase ELISA Kit | GPT elisa kit

Sheep Glutamic Pyruvic Transaminase ELISA Kit

Cat. Num. AAA738603

• Gene Names: GPT; AAT1; ALT1; GPT1
• Reactivity: Sheep
• Synonyms: Glutamic Pyruvic Transaminase; Sheep Glutamic Pyruvic Transaminase ELISA Kit; GPT elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 U/L.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPT elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This GPI/ALT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep GPI/ALT. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY GPI/ALT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPI/ALT antibody and an GPI/ALT-HRP conjugate. The assay sample and buffer are incubated together with GPI/ALT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPI/ALT concentration since GPI/ALT from samples and GPI/ALT-HRP conjugate compete for the anti-GPI/ALT antibody binding site. Since the number of sites is limited, as more sites are occupied by GPI/ALT from the sample, fewer sites are left to bind GPI/ALT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPI/ALT concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885351
• NCBI GeneID: 2875
• NCBI Accession #: NP_005300.1
• NCBI GenBank Nucleotide #: NM_005309.2
• Molecular Weight: 54,637 Da
• NCBI Official Full Name: alanine aminotransferase 1
• NCBI Official Synonym Full Names: glutamic-pyruvate transaminase (alanine aminotransferase)
• NCBI Official Symbol: GPT
• NCBI Official Synonym Symbols: AAT1; ALT1; GPT1
• NCBI Protein Information: alanine aminotransferase 1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1
• UniProt Protein Name: Alanine aminotransferase 1
• Protein Family: Alanine aminotransferase
• UniProt Gene Name: GPT
• UniProt Synonym Gene Names: AAT1; GPT1; ALT1; GPT 1
• UniProt Entry Name: ALAT1_HUMAN

NCBI Description

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

Uniprot Description

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; EC 2.6.1.2

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine catabolic process