PEAK1 recombinant protein

PEAK1, Unactive recombinant protein

Cat. Num. AAA515334

• Gene Names: PEAK1; SGK269
• Applications: Western Blot
• Purity: >95%
• Synonyms: PEAK1; Unactive recombinant protein; SGK269; PEAK1 recombinant protein

• Host: Sf9 insect cells
• Purity/Purification: >95%
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA. 0.25mM DTT, 0.1 mM PMSF, 25% glycerol.
• Concentration: 0.1ug/ul (varies by lot)
• Sequence Length: 11834

• Applicable Applications for PEAK1 recombinant protein: Kinase Assay, Western Blot (WB)
• Accession Number: NM 024776
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Recombinant human PEAK1 (1127-end) was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.; Product shipped on dry ice.; Stability: 1yr at -70°C from date of receipt.

SDS-PAGE

(The purity of PEAK1 was determined to be >95% by densitometry. Approx, MW: 100kDa)

Related Product Information for PEAK1 recombinant protein

Recombinant human PEAK1 (1127-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: PEAK1 or Pseudopodium-enriched atypical kinase 1 is a protein tyrosine kinase which belongs to NKF3 kinase family and regulates the cytoskeleton and has been shown to be involved in cancer progression (1). PEAK1 promotes anchorage-independent growth in the MDA-MB-435 human cancer cell line and promotes tumor progression of XPA-1 human pancreatic cancer cells when transplanted into the pancreas of nude mice. PEAK1 is highly expressed in spleen, followed by heart, ovary, brain, lung, kidney, and fetal brain (2).

Product Categories/Family for PEAK1 recombinant protein

Unactive Kinase; Signaling Proteins - Unactive Kinases

References

1. Wang, Y. et.al: Pseudopodium-enriched atypical kinase 1 regulates the cytoskeleton and cancer progression. Proc. Nat. Acad. Sci. 107: 10920-10925, 2010.
2. Ohara, O. et.al: Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method. DNA Res. 9: 47-57, 2002.

NCBI and Uniprot Product Information

• NCBI GI #: 336176090
• NCBI GeneID: 79834
• NCBI Accession #: NP_079052.2
• NCBI GenBank Nucleotide #: NM_024776
• UniProt Accession #: Q9H792; Q6ZS78; Q8NAZ4; Q8NCM3; Q8TEG7
• Molecular Weight: ~100 kDa
• NCBI Official Full Name: Homo sapiens NKF3 kinase family member (PEAK1), mRNA
• NCBI Official Symbol: PEAK1
• NCBI Official Synonym Symbols: SGK269
• NCBI Protein Information: pseudopodium-enriched atypical kinase 1; sugen kinase 269; tyrosine-protein kinase SgK269; pseudopodium enriched atypical kinase
• UniProt Protein Name: Pseudopodium-enriched atypical kinase 1
• Protein Family: Pseudopodium-enriched atypical kinase
• UniProt Gene Name: PEAK1
• UniProt Synonym Gene Names: KIAA2002
• UniProt Entry Name: PEAK1_HUMAN

NCBI Description

This gene encodes a non-receptor tyrosine kinase that is a member of the new kinase family three (NFK3) family. In migrating cells, the encoded protein is associated with the actin cytoskeleton and focal adhesions and promotes developing focal adhesion elongation. This protein may play a role in the regulation of cell migration, proliferation and cancer metastasis. [provided by RefSeq, Mar 2014]

Uniprot Description

Function: Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN. Ref.7

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.7

Subunit structure: Interacts with BCAR1 and CRK. Ref.7

Subcellular location: Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Note: Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation. Ref.7

Post-translational modification: Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro. Ref.7

Sequence similarities: Belongs to the protein kinase superfamily.Contains 1 protein kinase domain.

Sequence caution: The sequence BAB15006.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC87076.1 differs from that shown. Reason: Erroneous initiation.