Rabbit anti-Human, mouse HOMER3 Polyclonal Antibody | anti-HOMER3 antibody

HOMER3 Antibody (Center)

Cat. Num. AAA9206329

• Gene Names: HOMER3; VESL3; HOMER-3
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: HOMER3; Polyclonal Antibody; HOMER3 Antibody (Center); Homer protein homolog 3; Homer-3; anti-HOMER3 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This HOMER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-308 amino acids from the Central region of human HOMER3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.46 (varies by lot)
• Sequence Positions: 281-308
• Sequence Length: 325

• Applicable Applications for anti-HOMER3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of HOMER3 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane).HOMER3 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of HOMER3 Antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane).HOMER3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with HOMER3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SK-Br-3 cells using HOMER3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of HOMER3 (arrow) using rabbit polyclonal HOMER3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HOMER3 gene.)

Related Product Information for anti-HOMER3 antibody

HOMER3 is a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. This protein binds numerous other proteins including group I metabotropic glutamate receptors,inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.

Product Categories/Family for anti-HOMER3 antibody

Neuroscience

References

Norton,N., et.al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 120B (1), 18-21 (2003)

NCBI and Uniprot Product Information

• NCBI GI #: 224809410
• NCBI GeneID: 9454
• NCBI Accession #: NP_001139193.1; NP_001139194.1; NP_001139196.1; NP_004829.3
• NCBI GenBank Nucleotide #: NM_001145721.1
• UniProt Accession #: Q9NSC5; O14580; O95350; Q9NSB9; Q9NSC0; Q9NSC1; E9PCW9
• Molecular Weight: 39836
• NCBI Official Full Name: homer protein homolog 3 isoform 2
• NCBI Official Synonym Full Names: homer scaffolding protein 3
• NCBI Official Symbol: HOMER3
• NCBI Official Synonym Symbols: VESL3; HOMER-3
• NCBI Protein Information: homer protein homolog 3
• UniProt Protein Name: Homer protein homolog 3
• Protein Family: Homer protein
• UniProt Gene Name: HOMER3
• UniProt Synonym Gene Names: Homer-3
• UniProt Entry Name: HOME3_HUMAN

NCBI Description

This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

HOMER3: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses. Belongs to the Homer family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: postsynaptic membrane; cytoplasm; plasma membrane; cytosol; cell junction

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; metabotropic glutatmate receptor binding

Biological Process: metabotropic glutamate receptor signaling pathway; protein targeting