Western Blot (WB)
(The Cell lysates of HeLa and AGS (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate)
Mouse anti-Human KRT18 Monoclonal Antibody | anti-KRT18 antibody
KRT18 antibody
Recommended starting dilution is 1:500
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Cell lysates of HeLa and AGS (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate)
Western Blot (WB)
(The Cell lysates of HeLa and AGS (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate)
Immunohistochemistry (IHC)
(Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human KRT18 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).)
Immunohistochemistry (IHC)
(Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human KRT18 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).)
Immunofluorescence (IF)
(Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:500) with Texas Red (Red). ICC/IF analysis of KRT18 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:500) with Texas Red (Red). ICC/IF analysis of KRT18 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Research Articles on KRT18
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Product Notes
The KRT18 krt18 (Catalog #AAA200177) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KRT18 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB/IF: 1:250 ~ 1000. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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