Western Blot (WB)
(Western Blot detection against Immunogen (36.63kD).)
Mouse anti-Human PEAK1 Monoclonal Antibody | anti-PEAK1 antibody
PEAK1 (Pseudopodium-enriched Atypical Kinase 1, Sugen Kinase 269, Tyrosine-protein Kinase SgK269, KIAA2002, FLJ21140, FLJ34483)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Optimal dilutions to be determined by the researcher.
Western Blot (WB)
(Western Blot detection against Immunogen (36.63kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (36.63kD).)
Western Blot (WB)
(Western Blot analysis of PEAK1 expression in MCF-7.)
Western Blot (WB)
(Western Blot analysis of PEAK1 expression in MCF-7.)
Immunofluorescence (IF)
(Immunofluorescence analysis of PEAK1 in HeLa cells using MBS645902 (10ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence analysis of PEAK1 in HeLa cells using MBS645902 (10ug/ml).)
ELISA (EIA)
(Detection limit for recombinant GST tagged PEAK1 is ~0.3ng/ml using MBS645902 as a capture antibody in sandwich ELISA.)
ELISA (EIA)
(Detection limit for recombinant GST tagged PEAK1 is ~0.3ng/ml using MBS645902 as a capture antibody in sandwich ELISA.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a non-receptor tyrosine kinase that is a member of the new kinase family three (NFK3) family. In migrating cells, the encoded protein is associated with the actin cytoskeleton and focal adhesions and promotes developing focal adhesion elongation. This protein may play a role in the regulation of cell migration, proliferation and cancer metastasis. [provided by RefSeq, Mar 2014]
Uniprot Description
Function: Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN. Ref.7
Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.7
Subunit structure: Interacts with BCAR1 and CRK. Ref.7
Subcellular location: Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Note: Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation. Ref.7
Post-translational modification: Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro. Ref.7
Sequence similarities: Belongs to the protein kinase superfamily.Contains 1 protein kinase domain.
Sequence caution: The sequence BAB15006.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC87076.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on PEAK1
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Product Notes
The PEAK1 peak1 (Catalog #AAA645902) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PEAK1 (Pseudopodium-enriched Atypical Kinase 1, Sugen Kinase 269, Tyrosine-protein Kinase SgK269, KIAA2002, FLJ21140, FLJ34483) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PEAK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the PEAK1 peak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TPPPLPKKMI IRANTEPISK DLQKSMESSL CVMANPTYDI DPNWDASSAG SSISYELKGL DIESYDSLER PLRKERPVPS AANSISSLTT LSIKDRFSNS. It is sometimes possible for the material contained within the vial of "PEAK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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