Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA Kit | PDK4 elisa kit

Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA Kit

Cat. Num. AAA762474

• Reactivity: Human
• Synonyms: Pyruvate Dehydrogenase Kinase Isozyme 4; Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA Kit; PDK4/PDHK4/Pyruvate Dehydrogenase Kinase Isozyme 4; PDK4 elisa kit

• Reactivity: Human
• Sequence Length: 411

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PDK4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PDK4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PDK4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PDK4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PDK4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4505693
• NCBI GeneID: 5166
• NCBI Accession #: NP_002603.1
• NCBI GenBank Nucleotide #: NM_002612.3
• Molecular Weight: 46,469 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isozyme 4
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase 4
• NCBI Official Symbol: PDK4
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 4
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
• UniProt Gene Name: PDK4
• UniProt Synonym Gene Names: PDHK4
• UniProt Entry Name: PDK4_HUMAN

NCBI Description

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]

Uniprot Description

PDHK4: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK4 is abundant in heart, skeletal muscle, kidney, and pancreatic islets. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Mitochondrial; Protein kinase, atypical; EC 2.7.11.2; Kinase, protein; ATYPICAL group; PDHK family

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: ATP binding; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity

Biological Process: cellular metabolic process; cellular response to starvation; glucose homeostasis; glucose metabolic process; insulin receptor signaling pathway; positive regulation of defense response to virus by host; protein amino acid phosphorylation; pyruvate metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of bone resorption; regulation of fatty acid biosynthetic process; regulation of fatty acid oxidation; regulation of pH; response to starvation