Typical Testing Data/Standard Curve (for reference only)
Rabbit STAT1 ELISA Kit | STAT1 elisa kit
Rabbit STAT1 (Signal Transducer and Activator of Transcription 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to STAT1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for STAT1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain STAT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of STAT1. You can calculate the concentration of STAT1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.
Protein type: Transcription factor; DNA-binding
Cellular Component: nucleoplasm; axon; dendrite; cytoplasm; nuclear chromatin; nucleolus; nucleus
Molecular Function: identical protein binding; signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; DNA binding; protein phosphatase 2A binding; sequence-specific DNA binding; double-stranded DNA binding; tumor necrosis factor receptor binding; transcription factor activity; CCR5 chemokine receptor binding
Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; response to cAMP; blood circulation; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; response to lipopolysaccharide; signal transduction; response to exogenous dsRNA; tumor necrosis factor-mediated signaling pathway; regulation of transcription, DNA-dependent; negative regulation of viral protein levels in host cell; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; response to nutrient; caspase activation; response to drug; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; JAK-STAT cascade; negative regulation of angiogenesis; response to bacterium; cellular response to insulin stimulus; response to mechanical stimulus; response to cytokine stimulus; negative regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter
Research Articles on STAT1
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Product Notes
The Rabbit STAT1 stat1 (Catalog #AAA2511565) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2511565 ELISA Kit recognizes Rabbit STAT1. It is sometimes possible for the material contained within the vial of "STAT1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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