PA1 recombinant protein

PA1, 1-254aa, Human, His tag, E Coli

Cat. Num. AAA204030

• Gene Names: PAGR1; GAS; PA1; C16orf53
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: PA1; 1-254aa; Human; His tag; E Coli; PAXIP1-associated protein 1.; FLJ22459; GAS; MGC4606; C16orf53.; PA1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSLARGHGDT AASTAAPLSE EGEVTSGLQA LAVEDTGGPS ASAGKAEDEG EGGREETERE GSGGEEAQGE VPSAGGEEPA EEDSEDWCVP CSDEEVELPA DGQPWMPPPS EIQRLYELLA AHGTLELQAE ILPRRPPTPE AQSEEERSDE EPEAKEEEEE KPHMPTEFDF DDEPVTPKDS LIDRRRTPGS SARSQKREAR LDKVLSDMKR HKKLEEQILR TGRDLFSLDS EDPSPASPPL RSSGSSLFPR QRKY
• Sequence Length: 254

• Applicable Applications for PA1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PA1 recombinant protein

PA1 is a component of a Set1-like multiprotein histone methyltransferase complex. It interacts with PAXIP1/PTIP; the interaction is direct and is required for the association with the rest of the PTIP complex. This protein plays a critical role in maintaining genome stability, condensation of chromatin and progression through mitosis. Recombinant human PA1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PA1 recombinant protein

Signal Transduction

References

Liang J., et al. (2009) EMBO Rep. 10 (1):51-7. ; Cho YW., et al. (2007) J Biol Chem. 282 (28):20395-406.

NCBI and Uniprot Product Information

• NCBI GI #: 13375654
• NCBI GeneID: 79447
• NCBI Accession #: NP_078792
• NCBI GenBank Nucleotide #: NM_024516.3
• UniProt Accession #: Q9BTK6; A2ICR6
• Molecular Weight: 29.9 kDa (274aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: PAXIP1-associated glutamate-rich protein 1
• NCBI Official Synonym Full Names: PAXIP1 associated glutamate rich protein 1
• NCBI Official Symbol: PAGR1
• NCBI Official Synonym Symbols: GAS; PA1; C16orf53
• NCBI Protein Information: PAXIP1-associated glutamate-rich protein 1
• UniProt Protein Name: PAXIP1-associated glutamate-rich protein 1
• UniProt Gene Name: PAGR1
• UniProt Synonym Gene Names: C16orf53; PA1; GAS

NCBI Description

C16ORF53 (PA1) is a component of a Set1-like multiprotein histone methyltransferase complex (Cho et al., 2007 [PubMed 17500065]).[supplied by OMIM, May 2008]

Uniprot Description

Its association with the histone methyltransferase MLL2/MLL3 complex is suggesting a role in epigenetic transcriptional activation. However, in association with PAXIP1/PTIP is proposed to function at least in part independently of the MLL2/MLL3 complex. Proposed to be recruited by PAXIP1 to sites of DNA damage where the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage independently of the MLL2/MLL3 complex (PubMed:19124460). However, its function in DNA damage has been questioned (). During immunoglobulin class switching in activated B-cells is involved in transcription regulation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus independently of the MLL2/MLL3 complex (). Involved in both estrogen receptor-regulated gene transcription and estrogen-stimulated G1/S cell-cycle transition (PubMed:19039327). Acts as transcriptional cofactor for nuclear hormone receptors. Inhibits the induction properties of several steroid receptors such as NR3C1, AR and PPARG; the mechanism of inhibition appears to be gene-dependent (PubMed:23161582).