Rabbit anti-Human, Mouse PIAS4 Polyclonal Antibody | anti-PIAS4 antibody

PIAS4 Antibody

Cat. Num. AAA9601501

• Gene Names: PIAS4; PIASY; Piasg; ZMIZ6; PIAS-gamma
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: PIAS4; Polyclonal Antibody; PIAS4 Antibody; E3 SUMO protein ligase PIAS4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PAIASgamma; PIAS 4; PIAS gamma; PIAS-gamma; Pias4; PIAS4_HUMAN; PIASG; PIASgamma; PIASy; Protein inhibitor of activated STAT 4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; Protein inhibitor of activated STAT protein PIASy; Zinc finger MIZ type containing 6; ZMIZ 6; ZMIZ6; anti-PIAS4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIAS4 antibody detects endogenous levels of total PIAS4
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 510

• Applicable Applications for anti-PIAS4 antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human PIAS4
• Subcellular Location: Nucleus > PML Body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
• Tissue Specificity: Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
• Predicted Cross Reactivity: Pig, Bovine, Sheep, Dog, Chicken
• Similarity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%), Chicken (83%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9601501 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of PIAS4 expression in Jurkat cell extract.The lane on the left is treated with the antigen-specific peptide.)

Immunofluorescence (IF)

(MBS9601501 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-PIAS4 antibody

Function: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.
Subunit Structure: Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs) (By similarity). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G1 phase. Interacts with MTA1.
Post-translational Modifications: Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
Similarity: The LXXLL motif is a coregulator signature that is essential for transcriptional corepression. Belongs to the PIAS family.

NCBI and Uniprot Product Information

• NCBI GI #: 24850133
• NCBI GeneID: 51588
• NCBI Accession #: NP_056981.2
• NCBI GenBank Nucleotide #: NM_015897.3
• UniProt Accession #: Q8N2W9; O75926; Q96G19; Q9UN16
• Molecular Weight: Observed: 57 kDa; Predicted: 57 kDa
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS4
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT 4
• NCBI Official Symbol: PIAS4
• NCBI Official Synonym Symbols: PIASY; Piasg; ZMIZ6; PIAS-gamma
• NCBI Protein Information: E3 SUMO-protein ligase PIAS4
• UniProt Protein Name: E3 SUMO-protein ligase PIAS4
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: PIAS4
• UniProt Synonym Gene Names: PIASG; PIAS-gamma

Uniprot Description

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.