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SDS-Page

DENR recombinant protein

DENR, 1-198aa, Human, His tag, E Coli

Gene Names
DENR; DRP; DRP1; SMAP-3
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
DENR; 1-198aa; Human; His tag; E Coli; Density-regulated protein; DRP; DRP1; SMAP-3; DENR recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.15M NaCl.
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAADISESSG ADCKGDPRNS AKLDADYPLR VLYCGVCSLP TEYCEYMPDV AKCRQWLEKN FPNEFAKLTV ENSPKQEAGI SEGQGTAGEE EEKKKQKRGG RGQIKQKKKT VPQKVTIAKI PRAKKKYVTR VCGLATFEID LKEAQRFFAQ KFSCGASVTG EDEIIIQGDF TDDIIDVIQE KWPEVDDDSI EDLGEVKK
Sequence Length
198
Applicable Applications for DENR recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for DENR recombinant protein
DENR may be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. This protein plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1. Recombinant human DENR protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for DENR recombinant protein
References
Deyo JE., et al. (1998) DNA Cell Biol. 17 (5):437-47.; Oh JJ., et al. (1999) Nucleic Acids Res. 27 (20):4008-17.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24.3 kDa (218aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
NCBI Official Full Name
density-regulated protein
NCBI Official Synonym Full Names
density regulated re-initiation and release factor
NCBI Official Symbol
DENR
NCBI Official Synonym Symbols
DRP; DRP1; SMAP-3
NCBI Protein Information
density-regulated protein
UniProt Protein Name
Density-regulated protein
Protein Family
UniProt Gene Name
DENR
UniProt Synonym Gene Names
DRP1; DRP; SMAP-3

NCBI Description

This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants. [provided by RefSeq, Jul 2008]

Uniprot Description

May be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. Involved in translation initiation; promotes recruitmnet of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1.

Research Articles on DENR

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Product Notes

The DENR denr (Catalog #AAA204337) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's DENR can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the DENR denr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAADISESSG ADCKGDPRNS AKLDADYPLR VLYCGVCSLP TEYCEYMPDV AKCRQWLEKN FPNEFAKLTV ENSPKQEAGI SEGQGTAGEE EEKKKQKRGG RGQIKQKKKT VPQKVTIAKI PRAKKKYVTR VCGLATFEID LKEAQRFFAQ KFSCGASVTG EDEIIIQGDF TDDIIDVIQE KWPEVDDDSI EDLGEVKK. It is sometimes possible for the material contained within the vial of "DENR, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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