Rabbit EGR1 Monoclonal Antibody | anti-egr1 antibody

EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

Cat. Num. AAA608553

• Gene Names: egr1; krox24; krox-24; wu:fj64b05; wu:fq25f01
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: EGR1; Monoclonal Antibody; EGR1 (Zif268; Early Growth Response Protein 1; Krox-24; NGFI-A; TIS8; ZENK); Anti -EGR1 (Zif268; anti-egr1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9j16
• Specificity: Recognizes endogenous levels of total human EGR1 at ~75kD. Species Crossreactivity
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-egr1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunofluorescence (IF-IC): 1:800; Immunohistochemistry (Paraffin): 1:100; Immunoprecipitation: 1:50; Chromatin Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to the amino-terminal sequence of human EGR1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB analysis of extracts from serum starved PC12 cells, untreated or NGF-treated (100 ng/ml NGF for 2 hours) using MBS608553.)

Immunoprecipitation (IP)

(Chromatin immunoprecipitations were performed with cross-linked chromatin from 4x10e6 THP-1 cells treated with TPA (30ng/ml) overnight and either 10ul of MBS608553 or 2ul of Normal Rabbit IgG. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right), using MBS608553 (green) and MEK1/2 mouse mAb (red).)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human non-Hodgkin’s lymphoma using MBS608553.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain, using MBS608553.)

Related Product Information for anti-egr1 antibody

EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3).

Product Categories/Family for anti-egr1 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 18858601
• NCBI GeneID: 30498
• NCBI Accession #: NP_571323.1
• NCBI GenBank Nucleotide #: NM_131248.1
• UniProt Accession #: P26632
• Molecular Weight: 55,140 Da
• NCBI Official Full Name: early growth response protein 1
• NCBI Official Synonym Full Names: early growth response 1
• NCBI Official Symbol: egr1
• NCBI Official Synonym Symbols: krox24; krox-24; wu:fj64b05; wu:fq25f01
• NCBI Protein Information: early growth response protein 1; EGR-1; etID309970.14; zinc finger protein Krox-24
• UniProt Protein Name: Early growth response protein 1
• Protein Family: Early growth response protein
• UniProt Gene Name: egr1
• UniProt Synonym Gene Names: krox24
• UniProt Entry Name: EGR1_DANRE

Uniprot Description

Function: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation

Subcellular location: Nucleus.

Induction: By growth factors.

Sequence similarities: Belongs to the EGR C2H2-type zinc-finger protein family.Contains 3 C2H2-type zinc fingers.